Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa
by
Géraldine Le Goff 1,*, Philippe Lopes 1, Guillaume Arcile 1, Pinelopi Vlachou 2, Elsa Van Elslande 1
, Pascal Retailleau 1, Jean-François Gallard 1, Michal Weis 3, Yehuda Benayahu 3, Nikolas Fokialakis 2 and Jamal Ouazzani 1
Géraldine Le Goff 1,*, Philippe Lopes 1, Guillaume Arcile 1, Pinelopi Vlachou 2, Elsa Van Elslande 1, Pascal Retailleau 1, Jean-François Gallard 1, Michal Weis 3, Yehuda Benayahu 3, Nikolas Fokialakis 2 and Jamal Ouazzani 1
1
Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
2
Division of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece
3
School of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(12), 678; https://doi.org/10.3390/md17120678
Received: 8 November 2019 / Revised: 25 November 2019 / Accepted: 26 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Bioactive Marine Heterocyclic Compounds)
The fungi Chrysosporium lobatum TM-237-S5 was isolated from the sponge Acanthella cavernosa, collected from the mesophotic coral ecosystem of the Red Sea. The strain was cultivated on a potato dextrose agar (PDA) medium, coupling solid-state fermentation and solid-state extraction (SSF/SSE) with a neutral macroreticular polymeric adsorbent XAD Amberlite resin (AMBERLITE XAD1600N). The SSF/SSE lead to high chemodiversity and productivity compared to classical submerged cultivation. Ten phenalenone related compounds were isolated and fully characterized by one-dimensional and two-dimensional NMR and HRMS. Among them, four were found to be new compounds corresponding to isoconiolactone, (-)-peniciphenalenin F, (+)-8-hydroxyscleroderodin, and (+)-8-hydroxysclerodin. It is concluded that SSF/SSE is a powerful strategy, opening a new era for the exploitation of microbial secondary metabolites.
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Keywords:
solid-state fermentation; solid-state extraction; Chrysosporium lobatum; marine fungi; phenalenone derivatives
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MDPI and ACS Style
Le Goff, G.; Lopes, P.; Arcile, G.; Vlachou, P.; Van Elslande, E.; Retailleau, P.; Gallard, J.-F.; Weis, M.; Benayahu, Y.; Fokialakis, N.; Ouazzani, J. Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa. Mar. Drugs 2019, 17, 678.
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