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Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia

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Carl-von-Ossietzky University Oldenburg, Institute for Chemistry and Biology of the Marine Environment (ICBM), Schleusenstraße 1, D-26382 Wilhelmshaven, Germany
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Research Center for Oceanography LIPI, Jl. Pasir Putih Raya 1, Pademangan, Jakarta Utara 14430, Indonesia
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Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany
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TWINCORE-Centre for Experimental and Clinical Infection Research (Institute of Experimental Virology) Hannover. Feodor-Lynen-Str. 7-9, 30625 Hannover, Germany
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Department of Molecular and Medical Virology, Ruhr-University Bochum, 44801 Bochum, Germany
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Research Center for Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor KM 46, 16911 Cibinong, Indonesia
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Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Ammerländer Heerstrasse 231, D-26129 Oldenburg, Germany
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(11), 635; https://doi.org/10.3390/md17110635
Received: 8 October 2019 / Revised: 1 November 2019 / Accepted: 6 November 2019 / Published: 8 November 2019
(This article belongs to the Special Issue Marine Bacteria as Sources of Bioactive Compounds)
In order to minimize re-discovery of already known anti-infective compounds, we focused our screening approach on understudied, almost untapped marine environments including marine invertebrates and their associated bacteria. Therefore, two sea cucumber species, Holothuria leucospilota and Stichopus vastus, were collected from Lampung (Indonesia), and 127 bacterial strains were identified by partial 16S rRNA-gene sequencing analysis and compared with the NCBI database. In addition, the overall bacterial diversity from tissue samples of the sea cucumbers H. leucospilota and S. vastus was analyzed using the cultivation-independent Illumina MiSEQ analysis. Selected bacterial isolates were grown to high densities and the extracted biomass was tested against a selection of bacteria and fungi as well as the hepatitis C virus (HCV). Identification of putative bioactive bacterial-derived compounds were performed by analyzing the accurate mass of the precursor/parent ions (MS1) as well as product/daughter ions (MS2) using high resolution mass spectrometry (HRMS) analysis of all active fractions. With this attempt we were able to identify 23 putatively known and two previously unidentified precursor ions. Moreover, through 16S rRNA-gene sequencing we were able to identify putatively novel bacterial species from the phyla Actinobacteria, Proteobacteria and also Firmicutes. Our findings suggest that sea cucumbers like H. leucospilota and S. vastus are promising sources for the isolation of novel bacterial species that produce compounds with potentially high biotechnological potential. View Full-Text
Keywords: marine bacteria; sea cucumber; anti-infective marine derived compounds; de-replication; mass spectrometry marine bacteria; sea cucumber; anti-infective marine derived compounds; de-replication; mass spectrometry
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Wibowo, J.T.; Kellermann, M.Y.; Versluis, D.; Putra, M.Y.; Murniasih, T.; Mohr, K.I.; Wink, J.; Engelmann, M.; Praditya, D.F.; Steinmann, E.; Schupp, P.J. Biotechnological Potential of Bacteria Isolated from the Sea Cucumber Holothuria leucospilota and Stichopus vastus from Lampung, Indonesia. Mar. Drugs 2019, 17, 635.

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