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Open AccessArticle

Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells

1
Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea
2
Department of Marine Biotechnology, Gangneung-Wonju National University, Gangneung, Gangwon 25457, Korea
3
Department of Horticulture, Daegu Catholic University, Gyeongsan, Gyeongbuk 38430, Korea
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(10), 580; https://doi.org/10.3390/md17100580
Received: 1 September 2019 / Revised: 6 October 2019 / Accepted: 8 October 2019 / Published: 11 October 2019
(This article belongs to the Special Issue Marine Lipids with Biological Interest)
Arctoscopus japonicus is a cold-water marine fish. The present study investigated the fatty acid composition of A. japonicus egg lipids and their anti-inflammatory effects on LPS-stimulated RAW246.7 macrophages. The results showed that A. japonicus egg lipids contained primarily polyunsaturated fatty acids (52.9% of the total fatty acid content; mostly eicosapentaenoic acid [EPA, 21.2 ± 0.5%] and docosahexaenoic acid [DHA, 25.9 ± 0.1%]), followed by monounsaturated fatty acids and saturated fatty acids (23.7% and 23.4%, respectively). A. japonicus egg lipids significantly decreased nitric oxide (NO) production and suppressed the expression of immune-associated genes such as iNOS, COX-2, IL-1β, IL-6, and TNF-α LPS-stimulated RAW246.7 macrophages in dose-dependent manner. A. japonicus egg lipids also reduced the phosphorylation levels of NF-κB p-65, p38, ERK1/2, and JNK, key components of the NF-κB and MAPK pathways, suggesting that the lipid-induced anti-inflammatory activity is related to these signaling pathways. These results indicate that the lipids extracted from A. japonicus eggs have potential biofunctions and might be useful for regulating inflammation in macrophages. View Full-Text
Keywords: Arctoscopus japonicus; egg; lipid; anti-inflammation; macrophage; NF-κB; and MAPK Arctoscopus japonicus; egg; lipid; anti-inflammation; macrophage; NF-κB; and MAPK
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Rod-in, W.; Monmai, C.; Lee, S.-M.; Jung, S.-K.; You, S.; Park, W.J. Anti-Inflammatory Effects of Lipids Extracted from Arctoscopus japonicus Eggs on LPS-Stimulated RAW264.7 Cells. Mar. Drugs 2019, 17, 580.

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