Next Article in Journal
Enzymes from Marine Polar Regions and Their Biotechnological Applications
Previous Article in Journal
Magnificamide, a β-Defensin-Like Peptide from the Mucus of the Sea Anemone Heteractis magnifica, Is a Strong Inhibitor of Mammalian α-Amylases
Previous Article in Special Issue
Eckol Inhibits Particulate Matter 2.5-Induced Skin Keratinocyte Damage via MAPK Signaling Pathway
Open AccessArticle

Phlorofucofuroeckol A from Edible Brown Alga Ecklonia Cava Enhances Osteoblastogenesis in Bone Marrow-Derived Human Mesenchymal Stem Cells

1
Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea
2
Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Korea
3
Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan 49112, Korea
4
Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(10), 543; https://doi.org/10.3390/md17100543
Received: 14 August 2019 / Revised: 16 September 2019 / Accepted: 17 September 2019 / Published: 21 September 2019
(This article belongs to the Special Issue Characterization of Bioactive Components in Edible Algae)
The deterioration of bone formation is a leading cause of age-related bone disorders. Lack of bone formation is induced by decreased osteoblastogenesis. In this study, osteoblastogenesis promoting effects of algal phlorotannin, phlorofucofuroeckol A (PFF-A), were evaluated. PFF-A was isolated from brown alga Ecklonia cava. The ability of PFF-A to enhance osteoblast differentiation was observed in murine pre-osteoblast cell line MC3T3-E1 and human bone marrow-derived mesenchymal stem cells (huBM-MSCs). Proliferation and alkaline phosphatase (ALP) activity of osteoblasts during differentiation was assayed following PFF-A treatment along extracellular mineralization. In addition, effect of PFF-A on osteoblast maturation pathways such as Runx2 and Smads was analyzed. Treatment of PFF-A was able to enhance the proliferation of differentiating osteoblasts. Also, ALP activity was observed to be increased. Osteoblasts showed increased extracellular mineralization, observed by Alizarin Red staining, following PFF-A treatment. In addition, expression levels of critical proteins in osteoblastogenesis such as ALP, bone morphogenetic protein-2 (BMP-2), osteocalcin and β-catenin were stimulated after the introduction of PFF-A. In conclusion, PFF-A was suggested to be a potential natural product with osteoblastogenesis enhancing effects which can be utilized against bone-remodeling imbalances and osteoporosis-related complications. View Full-Text
Keywords: alkaline phosphatase; Ecklonia cava; phlorofucofuroeckol A; osteoblast; huBM-MSC alkaline phosphatase; Ecklonia cava; phlorofucofuroeckol A; osteoblast; huBM-MSC
Show Figures

Figure 1

MDPI and ACS Style

Oh, J.H.; Ahn, B.-N.; Karadeniz, F.; Kim, J.-A.; Lee, J.I.; Seo, Y.; Kong, C.-S. Phlorofucofuroeckol A from Edible Brown Alga Ecklonia Cava Enhances Osteoblastogenesis in Bone Marrow-Derived Human Mesenchymal Stem Cells. Mar. Drugs 2019, 17, 543.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop