Next Article in Journal
Asperlin Stimulates Energy Expenditure and Modulates Gut Microbiota in HFD-Fed Mice
Previous Article in Journal
Immunostimulatory Effects of Chitooligosaccharides on RAW 264.7 Mouse Macrophages via Regulation of the MAPK and PI3K/Akt Signaling Pathways
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Mar. Drugs 2019, 17(1), 37; https://doi.org/10.3390/md17010037

Comparative Analyses of Metabolomic Fingerprints and Cytotoxic Activities of Soft Corals from the Colombian Caribbean

1
Bioprospecting Research Group and Bioscience Doctoral Program, Faculty of Engineering, Campus Puente del Común, Universidad de La Sabana, 250001 Chía, Colombia
2
Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway (NUI Galway), University Road, H91 TK33 Galway, Ireland
3
Departamento de Química, Universidad Nacional de Colombia, Carrera 30 # 45-03, 111321 Bogotá, Colombia
4
Departamento de Ciencias Biológicas y Ambientales, Universidad Jorge Tadeo Lozano, Carrera 4 # 22-61, 110311 Bogotá, Colombia
*
Authors to whom correspondence should be addressed.
Received: 16 June 2018 / Revised: 20 December 2018 / Accepted: 28 December 2018 / Published: 9 January 2019
(This article belongs to the Special Issue Bioinformatics of Marine Natural Products)
Full-Text   |   PDF [2023 KB, uploaded 10 January 2019]   |  

Abstract

Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that inhabit various marine environments in tropical and subtropical areas. Several species are recognized as prolific sources of compounds with a wide array of biological activities. Recent advances in analytical techniques, supported by robust statistical analyses, have allowed the analysis and characterization of the metabolome present in a single living organism. In this study, a liquid chromatography-high resolution mass spectrometry metabolomic approach was applied to analyze the metabolite composition of 28 soft corals present in the Caribbean coast of Colombia. Multivariate data analysis was used to correlate the chemical fingerprints of soft corals with their cytotoxic activity against tumor cell lines for anticancer purpose. Some diterpenoids were identified as specific markers to discriminate between cytotoxic and non-cytotoxic crude extracts of soft corals against tumor cell lines. In the models generated from the comparative analysis of PLS-DA for tumor lines, A549 and SiHa, the diterpene 13-keto-1,11-dolabell-3(E),7(E),12(18)-triene yielded a high score in the variable importance in projection. These results highlight the potential of metabolomic approaches towards the identification of cytotoxic agents against cancer of marine origin. This workflow can be useful in several studies, mainly those that are time consuming, such as traditional bioprospecting of marine natural products. View Full-Text
Keywords: metabolomics; soft-corals; Pseudoplexaura flagellosa; diterpenes; cytotoxic activity; LC-HRMSs metabolomics; soft-corals; Pseudoplexaura flagellosa; diterpenes; cytotoxic activity; LC-HRMSs
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Santacruz, L.; Thomas, O.P.; Duque, C.; Puyana, M.; Tello, E. Comparative Analyses of Metabolomic Fingerprints and Cytotoxic Activities of Soft Corals from the Colombian Caribbean. Mar. Drugs 2019, 17, 37.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top