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Mar. Drugs 2018, 16(9), 342; https://doi.org/10.3390/md16090342

Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B

Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan
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Received: 24 August 2018 / Revised: 12 September 2018 / Accepted: 15 September 2018 / Published: 19 September 2018
(This article belongs to the Special Issue Marine Natural Products Discovery: In Honor of Late Prof. Tatsuo Higa)
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Abstract

The biosynthetic gene cluster for bisucaberin B (1, bsb gene cluster), an N-hydroxy-N-succinyl diamine (HSD)-based siderophore, was cloned from the marine bacterium Tenacibaculum mesophilum, originated from a marine sponge. The bsb gene cluster consists of six open reading frames (ORFs), in contrast to the four ORFs typically seen in biosynthetic gene clusters of the related molecules. Heterologous expression of the key enzyme, BsbD2, which is responsible for the final biosynthetic step of 1 resulted in production of bisucaberin B (1), but not bisucaberin (2) a macrocyclic counterpart of 1. To date, numbers of related enzymes producing macrocyclic analogues have been reported, but this work represents the first example of the HSD-based siderophore biosynthetic enzyme which exclusively produces a linear molecule rather than macrocyclic counterparts. View Full-Text
Keywords: siderophore; biosynthesis; heterologous production; bisucaberin; desferrioxamine siderophore; biosynthesis; heterologous production; bisucaberin; desferrioxamine
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Fujita, M.J.; Goto, Y.; Sakai, R. Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B. Mar. Drugs 2018, 16, 342.

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