Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation
Abstract
:1. Introduction
2. Results
2.1. PYP Pretreatment Attenuated PFOS-Induced Decrease in Viability of Frontal Cortical Neurons and the PYP-Induced Enhancement of Cell Viability Was Downregulated by Inhibiting TrkB Receptor
2.2. PYP Pretreatment Downregulated PFOS-Induced Increase in GRP78 Expression and Intracellular Calcium Levels in Frontal Cortical Neurons
2.3. PFOS-Induced GRP78 Expression Was Mediated by Phosphorylation of JNK Linked to CaMKII and PYP-Mediated Decrease in GRP78 Expression Was Carried Out by Activating TrkB Receptor-PI3K-ERK1/2 Signaling
2.4. PYP Pretreatment Downregulated the Increase in PFOS-Induced CaMKII and JNK Phosphorylation and Inhibition of CaMKII Attenuated the Increase in PFOS-Induced JNK Phosphorylation in Frontal Cortical Neurons
2.5. Microinjection of PYP Downregulated the Increase in PFOS-Induced ER Stress in Rat Prefrontal Cortex
3. Discussion
4. Materials and Methods
4.1. Peptide and Chemicals
4.2. Primary Cortical Neuron Culture
4.3. Cell Viability Assay
4.4. Double-Immunofluorescence Staining
4.5. Immunoblotting
4.6. Intracellular Calcium Level
4.7. Rat Surgery and Microinjection
4.8. Statistics
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Oh, J.H.; Kim, E.-Y.; Nam, T.-J. Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation. Mar. Drugs 2018, 16, 44. https://doi.org/10.3390/md16020044
Oh JH, Kim E-Y, Nam T-J. Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation. Marine Drugs. 2018; 16(2):44. https://doi.org/10.3390/md16020044
Chicago/Turabian StyleOh, Jeong Hwan, Eun-Young Kim, and Taek-Jeong Nam. 2018. "Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation" Marine Drugs 16, no. 2: 44. https://doi.org/10.3390/md16020044
APA StyleOh, J. H., Kim, E. -Y., & Nam, T. -J. (2018). Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation. Marine Drugs, 16(2), 44. https://doi.org/10.3390/md16020044