The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii
AbstractCystatins are a family of cysteine protease inhibitors which are associated with a variety of physiological and pathological processes in vivo. In the present study, the cDNA sequence of a cystatin F homologue called Lm-cystatin F was cloned from the buccal glands of Lampetra morii. Although Lm-cystatin F shares a lower homology with cystatin superfamily members, it is also composed of a signal peptide and three highly conserved motifs, including the G in the N-terminal, QXVXG, as well as the PW in the C-terminal of the sequence. After sequence optimization and recombination, the recombinant protein was expressed as a soluble protein in Escherichia coli with a molecular weight of 19.85 kDa. Through affinity chromatography and mass spectrometry analysis, the purified protein was identified as a recombinant Lm-cystatin F (rLm-cystatin F). Additionally, rLm-cystatin F could inhibit the activity of papain. Based on MTT assay, rLm-cystatin F inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) dose dependently with an IC50 of 5 μM. In vitro studies show that rLm-cystatin F suppressed the adhesion, migration, invasion, and tube formation of HUVECs, suggesting that rLm-cystatin F possesses anti-angiogenic activity, which provides information on the feeding mechanisms of Lampetra morii and insights into the application of rLm-cystatin F as a potential drug in the future. View Full-Text
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Zhu, M.; Li, B.; Wang, J.; Xiao, R. The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii. Mar. Drugs 2018, 16, 477.
Zhu M, Li B, Wang J, Xiao R. The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii. Marine Drugs. 2018; 16(12):477.Chicago/Turabian Style
Zhu, Mingru; Li, Bowen; Wang, Jihong; Xiao, Rong. 2018. "The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii." Mar. Drugs 16, no. 12: 477.
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