Next Article in Journal
Loss of Function in Zeaxanthin Epoxidase of Dunaliella tertiolecta Caused by a Single Amino Acid Mutation within the Substrate-Binding Site
Previous Article in Journal
A Novel Complex of Chitosan–Sodium Carbonate and Its Properties
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessReview
Mar. Drugs 2018, 16(11), 417; https://doi.org/10.3390/md16110417

Discovery Methodology of Novel Conotoxins from Conus Species

1
Key Laboratory of Tropical Biological Resources, Ministry of Education, Hainan University, Haikou 570228, China
2
Key Laboratory for Marine Drugs of Haikou, Hainan University, Haikou 570228, China
*
Author to whom correspondence should be addressed.
Received: 15 September 2018 / Revised: 21 October 2018 / Accepted: 25 October 2018 / Published: 30 October 2018
Full-Text   |   PDF [2204 KB, uploaded 30 October 2018]   |  

Abstract

Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various Conus species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well. View Full-Text
Keywords: discovery; methodology; novel conotoxins; crude venom purification; gene cloning; transcriptomics; proteomics; multi-omics discovery; methodology; novel conotoxins; crude venom purification; gene cloning; transcriptomics; proteomics; multi-omics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Fu, Y.; Li, C.; Dong, S.; Wu, Y.; Zhangsun, D.; Luo, S. Discovery Methodology of Novel Conotoxins from Conus Species. Mar. Drugs 2018, 16, 417.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top