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Open AccessArticle

Optimization of Extraction Conditions and Characterization of Pepsin-Solubilised Collagen from Skin of Giant Croaker (Nibea japonica)

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
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Author to whom correspondence should be addressed.
The authors contributed equally to this study and share first authorship.
Mar. Drugs 2018, 16(1), 29; https://doi.org/10.3390/md16010029
Received: 27 December 2017 / Revised: 6 January 2018 / Accepted: 10 January 2018 / Published: 14 January 2018
(This article belongs to the Special Issue Collagen from Marine Biological Source and Medical Applications)
In the present study, response surface methodology was performed to investigate the effects of extraction parameters on pepsin-solubilised collagen (PSC) from the skin of the giant croaker Nibea japonica. The optimum extraction conditions of PSC were as follows: concentration of pepsin was 1389 U/g, solid-liquid ratio was 1:57 and hydrolysis time was 8.67 h. Under these conditions, the extraction yield of PSC was up to 84.85%, which is well agreement with the predict value of 85.03%. The PSC from Nibea japonica skin was then characterized as type I collagen by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The fourier transforms infrared spetroscopy (FTIR) analysis revealed that PSC maintains its triple-helical structure by the hydrogen bond. All PSCs were soluble in the pH range of 1.0–4.0 and decreases in solubility were observed at neutral or alkaline conditions. All PSCs had a decrease in solubility in the presence of sodium chloride, especially with a concentration above 2%. So, the Nibea japonica skin could serve as another potential source of collagen. View Full-Text
Keywords: marine collagen; Nibea japonica; response surface methodology; optimization; characterization marine collagen; Nibea japonica; response surface methodology; optimization; characterization
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Yu, F.; Zong, C.; Jin, S.; Zheng, J.; Chen, N.; Huang, J.; Chen, Y.; Huang, F.; Yang, Z.; Tang, Y.; Ding, G. Optimization of Extraction Conditions and Characterization of Pepsin-Solubilised Collagen from Skin of Giant Croaker (Nibea japonica). Mar. Drugs 2018, 16, 29.

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