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Mar. Drugs 2017, 15(9), 294;

Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition

Shandong Academy of Pharmaceutical Science, Jinan 250101, China
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Shandong University of Traditional Chinese Medicine, Jinan 250355, China
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Authors to whom correspondence should be addressed.
Received: 25 July 2017 / Revised: 3 September 2017 / Accepted: 15 September 2017 / Published: 20 September 2017
(This article belongs to the Special Issue Marine Anti-inflammatory Agents)
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The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. View Full-Text
Keywords: THP-1 macrophages; anti-inflammatory; TLR4; NF-κB; MAPK; SPR analysis THP-1 macrophages; anti-inflammatory; TLR4; NF-κB; MAPK; SPR analysis

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Liu, F.; Zhang, X.; Li, Y.; Chen, Q.; Liu, F.; Zhu, X.; Mei, L.; Song, X.; Liu, X.; Song, Z.; Zhang, J.; Zhang, W.; Ling, P.; Wang, F. Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition. Mar. Drugs 2017, 15, 294.

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