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Mar. Drugs 2017, 15(9), 294; https://doi.org/10.3390/md15090294

Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition

1
Shandong Academy of Pharmaceutical Science, Jinan 250101, China
2
School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
3
School of Life Sciences, Lanzhou University, Lanzhou 730000, China
4
Shandong University of Traditional Chinese Medicine, Jinan 250355, China
5
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*
Authors to whom correspondence should be addressed.
Received: 25 July 2017 / Revised: 3 September 2017 / Accepted: 15 September 2017 / Published: 20 September 2017
(This article belongs to the Special Issue Marine Anti-inflammatory Agents)
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Abstract

The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. View Full-Text
Keywords: THP-1 macrophages; anti-inflammatory; TLR4; NF-κB; MAPK; SPR analysis THP-1 macrophages; anti-inflammatory; TLR4; NF-κB; MAPK; SPR analysis
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Liu, F.; Zhang, X.; Li, Y.; Chen, Q.; Liu, F.; Zhu, X.; Mei, L.; Song, X.; Liu, X.; Song, Z.; Zhang, J.; Zhang, W.; Ling, P.; Wang, F. Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition. Mar. Drugs 2017, 15, 294.

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