Next Article in Journal
Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years
Next Article in Special Issue
Effect of Salicornia herbacea on Osteoblastogenesis and Adipogenesis in Vitro
Previous Article in Journal
Bioactive Compounds from Macroalgae in the New Millennium: Implications for Neurodegenerative Diseases
Previous Article in Special Issue
Production of Avaroferrin and Putrebactin by Heterologous Expression of a Deep-Sea Metagenomic DNA
Open AccessArticle

Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species

1
Postgraduate Program in Pharmaceutical Sciences, State University of Maringa, Colombo Avenue 5790, Maringa, Parana CEP 87020-900, Brazil
2
Department of Marine Biology, Federal Fluminense University, P.O. Box 100644, Niteroi, Rio de Janeiro CEP 24001-970, Brazil
3
Department of Basic Health Sciences, State University of Maringa, Colombo Avenue 5790, Maringa, Parana CEP 87020-900, Brazil
*
Author to whom correspondence should be addressed.
Mar. Drugs 2014, 12(9), 4973-4983; https://doi.org/10.3390/md12094973
Received: 24 January 2014 / Revised: 25 March 2014 / Accepted: 23 April 2014 / Published: 25 September 2014
(This article belongs to the Special Issue Advances and New Perspectives in Marine Biotechnology)
Chagas’ disease, a vector-transmitted infectious disease, is caused by the protozoa parasite Trypanosoma cruzi. Drugs that are currently available for the treatment of this disease are unsatisfactory, making the search for new chemotherapeutic agents a priority. We recently described the trypanocidal action of (−)-elatol, extracted from the macroalga Laurencia dendroidea. However, nothing has been described about the mechanism of action of this compound on amastigotes that are involved in the chronic phase of Chagas’ disease. The goal of the present study was to evaluate the effect of (−)-elatol on the formation of superoxide anions (O2), DNA fragmentation, and autophagy in amastigotes of T. cruzi to elucidate the possible mechanism of the trypanocidal action of (−)-elatol. Treatment of the amastigotes with (−)-elatol increased the formation of O2•− at all concentrations of (−)-elatol assayed compared with untreated parasites. Increased fluorescence was observed in parasites treated with (−)-elatol, indicating DNA fragmentation and the formation of autophagic compartments. The results suggest that the trypanocidal action of (−)-elatol might involve the induction of the autophagic and apoptotic death pathways triggered by an imbalance of the parasite’s redox metabolism. View Full-Text
Keywords: (−)-elatol; Laurencia dendroidea; Trypanosoma cruzi; Chagas’ disease; reactive oxygen species (−)-elatol; Laurencia dendroidea; Trypanosoma cruzi; Chagas’ disease; reactive oxygen species
Show Figures

Figure 1

MDPI and ACS Style

Desoti, V.C.; Lazarin-Bidóia, D.; Sudatti, D.B.; Pereira, R.C.; Ueda-Nakamura, T.; Nakamura, C.V.; De Oliveira Silva, S. Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species. Mar. Drugs 2014, 12, 4973-4983.

AMA Style

Desoti VC, Lazarin-Bidóia D, Sudatti DB, Pereira RC, Ueda-Nakamura T, Nakamura CV, De Oliveira Silva S. Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species. Marine Drugs. 2014; 12(9):4973-4983.

Chicago/Turabian Style

Desoti, Vânia C.; Lazarin-Bidóia, Danielle; Sudatti, Daniela B.; Pereira, Renato C.; Ueda-Nakamura, Tania; Nakamura, Celso V.; De Oliveira Silva, Sueli. 2014. "Additional Evidence of the Trypanocidal Action of (−)-Elatol on Amastigote Forms through the Involvement of Reactive Oxygen Species" Mar. Drugs 12, no. 9: 4973-4983.

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop