Localization and Characterization of Ferritin in Demospongiae: A Possible Role on Spiculogenesis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analysis of Intracellular Structures
2.2. One Dimensional Gel Electrophoresis and Western Blotting Analysis of Ferritin in S. domuncula and Primmorph Tissues
2.3. Sequence Alignment
2.4. Immunohistology of S. domuncula Filaments/Spicules and Tissue
2.5. Elemental Analysis (ICP-MS) of S. domuncula Filaments
2.6. Magnetic Resonance Imaging (MRI) of S. domuncula Filaments and Spicules
3. Discussion
4. Experimental Section
4.1. Primmorphs Cell Culture
4.2. Sample Preparation and Microscopy Analysis
4.3. Protein Extraction
4.4. One-Dimensional Gel Electrophoresis and Western Immunoblotting
4.5. Sequence Analysis of Ferritin Deduced Protein
4.6. Preparation of Suberites Domuncula Spicules and Axial Filaments
4.7. Histological Preparation of Suberites Domuncula Tissue
4.8. Immunohistology of Suberites Domuncula Filaments, Spicules and Tissue
4.9. Induced Couple Plasma—Mass Spectrometry (ICP-MS) Analysis of S. domuncula Filaments
4.10. MRI Measurements, Imaging and Computer Tomography (CT)
5. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Natalio, F.; Wiese, S.; Friedrich, N.; Werner, P.; Tahir, M.N. Localization and Characterization of Ferritin in Demospongiae: A Possible Role on Spiculogenesis. Mar. Drugs 2014, 12, 4659-4676. https://doi.org/10.3390/md12084659
Natalio F, Wiese S, Friedrich N, Werner P, Tahir MN. Localization and Characterization of Ferritin in Demospongiae: A Possible Role on Spiculogenesis. Marine Drugs. 2014; 12(8):4659-4676. https://doi.org/10.3390/md12084659
Chicago/Turabian StyleNatalio, Filipe, Stefanie Wiese, Norman Friedrich, Peter Werner, and Muhammad Nawaz Tahir. 2014. "Localization and Characterization of Ferritin in Demospongiae: A Possible Role on Spiculogenesis" Marine Drugs 12, no. 8: 4659-4676. https://doi.org/10.3390/md12084659