Identification of Marine Neuroactive Molecules in Behaviour-Based Screens in the Larval Zebrafish
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry
2.2. Neuroactive Activity of 36 Compounds were Evaluated in Zebrafish Behavioral Assay
2.3. Neuroactive Compounds Exhibited Different Behavioral Patterns
2.4. Compounds 1 and 37–41 Suppressed Locomotor Activity in Larval Zebrafish
2.5. The Potential of Selected Compounds for New Antiepileptic Drug Development in PTZ-Induced Seizure Model
2.6. The Dose-Response Study of Compound 40 in PTZ Model
3. Experimental Section
3.1. Chemistry
3.2. General Procedure of Synthesizing Compounds 9–12; Take 9 for Example
3.2.1. Compound 9
3.2.2. Compound 10
3.2.3. Compound 11
3.2.4. Compound 12
3.3. Zebrafish Behavior-Based Activity Screen
3.3.1. Zebrafish Maintenance
3.3.2. Zebrafish Behavioral Assay
3.3.3. Morphology Assay
3.3.4. Quantitative RT Real Time PCR
- c-fos Forward primer: 5′-GCTCCATCTCAGTCCCAGAG-3′
- c-fos Reverse primer: 5′-AGAGTGGGCTCCAGATCAGA-3′
- β-actin Forward primer: 5′-CCGTTGCCCCGAGGCTCTCT-3′
- β-actin Reverse primer: 5′-CGCATCCTGAGTCAATGCGCCA-3′
3.3.5. Data Analysis
4. Conclusions
Abbreviations
HT | high-throughput |
PTZ | pentylenetetrazol |
GABA | γ-Aminobutyric acid |
5-HT | 5-hydroxy-tryptamine |
NMDA | N-Methyl-d-aspartic acid |
CBZ | carbamazepine |
DMSO | dimethyl sulfoxide |
dpf | days post-fertilization |
hpf | hours post fertilization |
CNS | central nervous system |
IEGs | immediate early genes |
EEG | electroencephalography |
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Long, S.-M.; Liang, F.-Y.; Wu, Q.; Lu, X.-L.; Yao, X.-L.; Li, S.-C.; Li, J.; Su, H.; Pang, J.-Y.; Pei, Z. Identification of Marine Neuroactive Molecules in Behaviour-Based Screens in the Larval Zebrafish. Mar. Drugs 2014, 12, 3307-3322. https://doi.org/10.3390/md12063307
Long S-M, Liang F-Y, Wu Q, Lu X-L, Yao X-L, Li S-C, Li J, Su H, Pang J-Y, Pei Z. Identification of Marine Neuroactive Molecules in Behaviour-Based Screens in the Larval Zebrafish. Marine Drugs. 2014; 12(6):3307-3322. https://doi.org/10.3390/md12063307
Chicago/Turabian StyleLong, Si-Mei, Feng-Yin Liang, Qi Wu, Xi-Lin Lu, Xiao-Li Yao, Shi-Chang Li, Jing Li, Huanxing Su, Ji-Yan Pang, and Zhong Pei. 2014. "Identification of Marine Neuroactive Molecules in Behaviour-Based Screens in the Larval Zebrafish" Marine Drugs 12, no. 6: 3307-3322. https://doi.org/10.3390/md12063307