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Open AccessArticle

Simple and Rapid Quality Control of Sulfated Glycans by a Fluorescence Sensor Assay—Exemplarily Developed for the Sulfated Polysaccharides from Red Algae Delesseria sanguinea

Pharmaceutical Institute, Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, D-24118 Kiel, Germany
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Mar. Drugs 2014, 12(4), 2205-2227; https://doi.org/10.3390/md12042205
Received: 26 January 2014 / Revised: 28 February 2014 / Accepted: 24 March 2014 / Published: 10 April 2014
(This article belongs to the Special Issue Marine Glycoconjugates)
Sulfated polysaccharides (SP) from algae are of great interest due to their manifold biological activities. Obstacles to commercial (especially medical) application include considerable variability and complex chemical composition making the analysis and the quality control challenging. The aim of this study was to evaluate a simple microplate assay for screening the quality of SP. It is based on the fluorescence intensity (FI) increase of the sensor molecule Polymer-H by SP and was originally developed for direct quantification of SP. Exemplarily, 65 SP batches isolated from the red alga Delesseria sanguinea (D.s.-SP) and several other algae polysaccharides were investigated. Their FI increase in the Polymer-H assay was compared with other analytical parameters. By testing just one concentration of a D.s.-SP sample, quality deviations from the reference D.s.-SP and thus both batch-to-batch variability and stability can be detected. Further, structurally distinct SP showed to differ in their concentration-dependent FI profiles. By using corresponding reference compounds, the Polymer-H assay is therefore applicable as identification assay with high negative predictability. In conclusion, the Polymer-H assay showed to represent not only a simple method for quantification, but also for characterization identification and differentiation of SP of marine origin. View Full-Text
Keywords: Delesseria sanguinea; sulfated polysaccharides; quality control; fluorescence sensor; (red) algae; biopolymer; assay development; Polymer-H; sulfated glycan; fondaparinux Delesseria sanguinea; sulfated polysaccharides; quality control; fluorescence sensor; (red) algae; biopolymer; assay development; Polymer-H; sulfated glycan; fondaparinux
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Lühn, S.; Grimm, J.C.; Alban, S. Simple and Rapid Quality Control of Sulfated Glycans by a Fluorescence Sensor Assay—Exemplarily Developed for the Sulfated Polysaccharides from Red Algae Delesseria sanguinea. Mar. Drugs 2014, 12, 2205-2227.

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