Design and Synthesis of Pro-Apoptotic Compounds Inspired by Diatom Oxylipins
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inhibition of Cell Division by Natural Oxylipins
2.2. Synthesis of Oxylipin Analogues and Evaluation of Inhibitory Activity on Development of Sea-Urchin Embryos
2.3. Apoptotic Activity of Compound 22
3. Experimental Section
3.1. Chemical Synthesis of Oxylipin Analogues
3.2. Sea Urchin Gamete Collection
3.3. Antimitotic Assay on Sea Urchin Embryos
3.4. Tunel Fluorescence Labeling (TUNEL)
3.5. Cell Culture and Cell Viability Assay
3.6. Annexin V Assay
3.7. Caspase Assay
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Abbreviations
DIBAL | diisobutylaluminum hydride |
THF | tetrahydrofuran |
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Romano, G.; Manzo, E.; Russo, G.L.; D'Ippolito, G.; Cutignano, A.; Russo, M.; Fontana, A. Design and Synthesis of Pro-Apoptotic Compounds Inspired by Diatom Oxylipins. Mar. Drugs 2013, 11, 4527-4543. https://doi.org/10.3390/md11114527
Romano G, Manzo E, Russo GL, D'Ippolito G, Cutignano A, Russo M, Fontana A. Design and Synthesis of Pro-Apoptotic Compounds Inspired by Diatom Oxylipins. Marine Drugs. 2013; 11(11):4527-4543. https://doi.org/10.3390/md11114527
Chicago/Turabian StyleRomano, Giovanna, Emiliano Manzo, Gian Luigi Russo, Giuliana D'Ippolito, Adele Cutignano, Maria Russo, and Angelo Fontana. 2013. "Design and Synthesis of Pro-Apoptotic Compounds Inspired by Diatom Oxylipins" Marine Drugs 11, no. 11: 4527-4543. https://doi.org/10.3390/md11114527