Pseudoalteromone B: A Novel 15C Compound from a Marine Bacterium Pseudoalteromonas sp. CGH2XX
2. Results and Discussion
|Position||δH (J in Hz)||δC, Mult.|
|1||2.18 s||31.9, CH3|
|3a/b||2.58 d (17.2); 2.65 d (17.2)||52.3, CH2|
|5||1.51 m||41.9, CH2|
|6||2.04 m||22.5, CH2|
|7||5.09 tq (7.2, 1.2)||124.8, CH|
|9||1.96 t (7.2)||38.8, CH2|
|10||1.66 quintet (7.2)||21.8, CH2|
|11||2.37 t (7.2)||43.0, CH2|
|13||2.12 s||29.9, CH3|
|14||1.22 s||26.7, CH3|
|15||1.58 br s||15.7, CH3|
3. Experimental Section
3.1. General Experimental Procedures
3.2. Marine Bacteria Isolation, Culture Conditions and Extract Preparation
3.4. Cytotoxicity Testing
3.5. Elastase Release by Human Neutrophils
References and Notes
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- The authors regret that there is an error in pages 1 and 2 of  (pages 1675 and 1676 of the issue). In , the ubiquinone, pseudoalteromone A, was reported to display an inhibitory effect on the release of elastase (inhibition rate 45.1%) by human nuetrophils at a concentration of 10 µg/mL. However, after detailed collating, we found this data was cited incorrectly. The data (inhibition rate 45.1%) expressed an inhibitory effect of an organic extract from the marine bacterium Pseudoalteromonas sp. CGH2XX on the release of elastase by human nuetrophils as presented in this study. The in vitro anti-inflammatory effects of pseudoalteromone A were tested again. Pseudoalteromone A displayed moderately inhibitory effects on the generation of superoxide anion and the release of elastase (inhibition rates 38.0% and 20.2%) by human neutrophils at a concentration of 10 µg/mL. Diphenyl indonium (DPI) and elastatinal were used as reference compounds in anti-inflammatory activity testing. DPI displayed an inhibitory effect on superoxide anion generation (IC50 = 0.9 µg/mL), and elastatinal exhibited an inhibitory effect on elastase release (IC50 = 31.9 µg/mL) by human neutrophils, respectively. The authors apologize for any inconvenience caused by this error.
- Samples Availability: Not available.
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