Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mere15 Inhibits K562 Cell Proliferation
2.2. Mere15 Induces Apoptosis in K562 Cells
2.3. Mere15 Induces ROS Generation
2.4. Mere15 Disturbs MMP
2.5. Mere15 Induces a G0/G1 Phase Arrest
2.6. Mere15 Inhibits Tubulin Polymerization both in K562 Cells and in Cell Free System
2.7. Mere15 Interacts with Tubulin in Vitro
3. Experimental Section
3.1. Materials and Reagents
3.2. Cell Lines and Cell Culture
3.3. Cell Viability Assessment
3.4. Hoechst33342/Propidium Iodide (PI) Dual Staining Assays
3.5. Measurement of Reactive Oxygen Species (ROS) Generation
3.6. Assessment of Mitochondrial Membrane Potential (MMP)
3.7. Cell Cycle Analysis
3.8. Immunofluorescence Detection of Cellular Microtubule
3.9. Tubulin Polymerization Assay
3.10. Intrinsic Tryptophan Fluorescence of Tubulin
3.11. Hydrophobic Analysis of Tubulin Using Bis-ANS
3.12. Data Analysis
4. Conclusions
Acknowledgments
References
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Liu, M.; Zhao, X.; Zhao, J.; Xiao, L.; Liu, H.; Wang, C.; Cheng, L.; Wu, N.; Lin, X. Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus. Mar. Drugs 2012, 10, 2596-2607. https://doi.org/10.3390/md10112596
Liu M, Zhao X, Zhao J, Xiao L, Liu H, Wang C, Cheng L, Wu N, Lin X. Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus. Marine Drugs. 2012; 10(11):2596-2607. https://doi.org/10.3390/md10112596
Chicago/Turabian StyleLiu, Ming, Xiangzhong Zhao, Jin Zhao, Lin Xiao, Haizhou Liu, Cuicui Wang, Linyou Cheng, Ning Wu, and Xiukun Lin. 2012. "Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus" Marine Drugs 10, no. 11: 2596-2607. https://doi.org/10.3390/md10112596
APA StyleLiu, M., Zhao, X., Zhao, J., Xiao, L., Liu, H., Wang, C., Cheng, L., Wu, N., & Lin, X. (2012). Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus. Marine Drugs, 10(11), 2596-2607. https://doi.org/10.3390/md10112596