Epigenetic Marks, DNA Damage Markers, or Both? The Impact of Desiccation and Accelerated Aging on Nucleobase Modifications in Plant Genomic DNA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Treatments
2.2. Viability Assessment
2.3. ROS Detection
2.4. Measurement of Total and Non-Protein Antioxidant Capacity
2.5. DNA Isolation and Measurement of DNA Modifications
2.6. Statistical Analysis
3. Results
3.1. Explant Viability: TTC Assay and Regrowth
3.2. Measurement of Reactive Oxygen Species
3.3. Measurement of Total Antioxidant Capacity and Non-Protein Antioxidant Capacity
3.4. Assessment of the Genomic Level of m5C, 8-oxoG, and hm5C in the DNA of Embryonic Axes of Acer pseudoplatanus
3.5. Correlation and Principal Component Analyses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Desiccation (h) | MC, % (WC, g H2O g−1 Dry Weight) |
---|---|
0 | 50.2 ± 0.08 |
(1.01 ± 0.03) | |
1 | 19.3 ± 0.07 |
(0.24 ± 0.01) | |
4 | 11.9 ± 0.08 |
(0.13 ± 0.01) | |
6 | 9.2 ± 0.4 |
(0.10 ± 0.01) | |
18 | 5.7 ± 1.2 |
(0.06 ± 0.01) |
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Plitta-Michalak, B.P.; Litkowiec, M.; Michalak, M. Epigenetic Marks, DNA Damage Markers, or Both? The Impact of Desiccation and Accelerated Aging on Nucleobase Modifications in Plant Genomic DNA. Cells 2022, 11, 1748. https://doi.org/10.3390/cells11111748
Plitta-Michalak BP, Litkowiec M, Michalak M. Epigenetic Marks, DNA Damage Markers, or Both? The Impact of Desiccation and Accelerated Aging on Nucleobase Modifications in Plant Genomic DNA. Cells. 2022; 11(11):1748. https://doi.org/10.3390/cells11111748
Chicago/Turabian StylePlitta-Michalak, Beata P., Monika Litkowiec, and Marcin Michalak. 2022. "Epigenetic Marks, DNA Damage Markers, or Both? The Impact of Desiccation and Accelerated Aging on Nucleobase Modifications in Plant Genomic DNA" Cells 11, no. 11: 1748. https://doi.org/10.3390/cells11111748