Retrospective Serologic Assessment of Vitamin D Levels in Children from Western Romania: A Cross-Sectional Study
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design, Population and Data Collection
2.2. Inclusion and Exclusion Criteria
2.3. Sample Collection and Laboratory Assessments
2.4. Data and Statistical Analysis
2.5. Ethical Approval
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Bouftas, F.; DeVries, C. Vitamin D and pediatric bone health: Important information and considerations for the pediatric orthopaedic surgeon. J. Pediatr. Orthop. Soc. N. Am. 2024, 7, 100042. [Google Scholar] [CrossRef]
- Chauhan, K.; Shahrokhi, M.; Huecker, M.R. Vitamin D. In StatPearls; StatPearls Publishing: Treasure Island, FL, USA, 2024. [Google Scholar]
- Allgrove, J. Physiology of calcium, phosphate and magnesium. Endocr. Dev. 2009, 16, 8–31. [Google Scholar] [CrossRef] [PubMed]
- Samuel, S.; Sitrin, M.D. Vitamin D’s role in cell proliferation and differentiation. Nutr. Rev. 2008, 66, S116–S124. [Google Scholar] [CrossRef] [PubMed]
- Ganmaa, D.; Bromage, S.; Khudyakov, P.; Erdenenbaatar, S.; Delgererekh, B.; Martineau, A.R. Influence of Vitamin D Supplementation on Growth, Body Composition, and Pubertal Development Among School-aged Children in an Area With a High Prevalence of Vitamin D Deficiency: A Randomized Clinical Trial. JAMA Pediatr. 2023, 177, 32–41. [Google Scholar] [CrossRef]
- Charoenngam, N.; Holick, M.F. Immunologic Effects of Vitamin D on Human Health and Disease. Nutrients 2020, 12, 2097. [Google Scholar] [CrossRef]
- Benameur, T. Seasonal Variations in 25-Hydroxyvitamin D Levels among Pediatric Patients Attending the Healthcare Centre. Nutrients 2024, 16, 379. [Google Scholar] [CrossRef]
- Aranow, C. Vitamin D and the immune system. J. Investig. Med. Off. Publ. Am. Fed. Clin. Res. 2011, 59, 881–886. [Google Scholar] [CrossRef]
- Adams, J.S.; Hewison, M. Update in vitamin D. J. Clin. Endocrinol. Metab. 2010, 95, 471–478. [Google Scholar] [CrossRef]
- DeLuca, H.F. Overview of general physiologic features and functions of vitamin D. Am. J. Clin. Nutr. 2004, 80, 1689S–1696S. [Google Scholar] [CrossRef]
- Holick, M.F. Shedding new light on the role of the sunshine vitamin D for skin health: The lncRNA-skin cancer connection. Exp. Dermatol. 2014, 23, 391–392. [Google Scholar] [CrossRef]
- Raiten, D.J.; Picciano, M.F. Vitamin D and health in the 21st century: Bone and beyond. Executive summary. Am. J. Clin. Nutr. 2004, 80, 1673S–1677S. [Google Scholar] [CrossRef] [PubMed]
- Reid, I.R.; Bolland, M.J.; Grey, A. Effects of vitamin D supplements on bone mineral density: A systematic review and meta-analysis. Lancet 2014, 383, 146–155. [Google Scholar] [CrossRef] [PubMed]
- Stroud, M.L.; Stilgoe, S.; Stott, V.E.; Alhabian, O.; Salman, K. Vitamin D—A review. Aust. Fam. Physician 2008, 37, 1002–1005. [Google Scholar] [PubMed]
- Holick, M.F. Sunlight, ultraviolet radiation, vitamin D and skin cancer: How much sunlight do we need? Adv. Exp. Med. Biol. 2014, 810, 19–36. [Google Scholar]
- Holick, M.F. Environmental factors that influence the cutaneous production of vitamin D. Am. J. Clin. Nutr. 1995, 61, 638S–645S. [Google Scholar] [CrossRef]
- Wacker, M.; Holick, M.F. Sunlight and Vitamin D. Dermatoendocrinology 2013, 5, 51–108. [Google Scholar] [CrossRef]
- Horton-French, K.; Dunlop, E.; Lucas, R.M.; Pereira, G.; Black, L.J. Prevalence and predictors of vitamin D deficiency in a nationally representative sample of Australian adolescents and young adults. Eur. J. Clin. Nutr. 2021, 75, 1627–1636. [Google Scholar] [CrossRef]
- Malacova, E.; Cheang, P.R.; Dunlop, E.; Sherriff, J.L.; Lucas, R.M.; Daly, R.M.; Nowson, C.A.; Black, L.J. Prevalence and predictors of vitamin D deficiency in a nationally representative sample of adults participating in the 2011–2013 Australian Health Survey. Br. J. Nutr. 2019, 121, 894–904. [Google Scholar] [CrossRef]
- Zhu, K.; Oddy, W.H.; Holt, P.; Ping-Delfos, W.C.S.; Mountain, J.; Lye, S.; Pennell, C.; Hart, P.H.; Walsh, J.P. Tracking of vitamin D status from childhood to early adulthood and its association with peak bone mass. Am. J. Clin. Nutr. 2017, 106, 276–283. [Google Scholar] [CrossRef]
- Jamatia, E.; Roy, S.; Das, A.; Dewan, P.; Debnath, T. Vitamin D status of children at a tertiary care hospital of Agartala, North-east India: A cross-sectional study. Indian J. Health Sci. Biomed. Res. Kleu 2022, 15, 214. [Google Scholar] [CrossRef]
- Alonso, M.A.; Mantecón, L.; Santos, F. Vitamin D deficiency in children: A challenging diagnosis! Pediatr. Res. 2019, 85, 596–601. [Google Scholar] [CrossRef] [PubMed]
- Kumar, J.; Muntner, P.; Kaskel, F.J.; Hailpern, S.M.; Melamed, M.L. Prevalence and associations of 25-hydroxyvitamin D deficiency in US children: NHANES 2001-2004. Pediatrics 2009, 124, e362–e370. [Google Scholar] [CrossRef] [PubMed]
- Absoud, M.; Cummins, C.; Lim, M.J.; Wassmer, E.; Shaw, N. Prevalence and predictors of vitamin D insufficiency in children: A Great Britain population based study. PLoS ONE 2011, 6, e22179. [Google Scholar] [CrossRef] [PubMed]
- Andıran, N.; Çelik, N.; Akça, H.; Doğan, G. Vitamin D deficiency in children and adolescents. J. Clin. Res. Pediatr. Endocrinol. 2012, 4, 25–29. [Google Scholar] [CrossRef] [PubMed]
- Tiwari, L.; Puliyel, J.M. Vitamin D level in slum children of Delhi. Indian Pediatr. 2004, 41, 1076–1077. [Google Scholar]
- Avagyan, D.; Neupane, S.P.; Gundersen, T.E.; Madar, A.A. Vitamin D status in pre-school children in rural Nepal. Public Health Nutr. 2016, 19, 470–476. [Google Scholar] [CrossRef]
- Shaka, M.F.; Hussen Kabthymer, R.; Meshesha, M.D.; Borde, M.T. Vitamin D deficiency among apparently healthy children and children with common medical illnesses in Sub-Saharan Africa: A systematic review and meta-analysis. Ann. Med. Surg. 2012 2022, 75, 103403. [Google Scholar] [CrossRef]
- Badiu Tișa, I.; Cozma-Petruț, A.; Samașca, G.; Miere, D.; Filip, L.; Banc, R.; Mîrza, O.; Iancu, M. Vitamin D Status among 2-18-Year-Old Romanian Pediatric Patients: A Single-Center Study. Nutrients 2024, 16, 2266. [Google Scholar] [CrossRef]
- Chirita-Emandi, A.; Socolov, D.; Haivas, C.; Calapiș, A.; Gheorghiu, C.; Puiu, M. Vitamin D Status: A Different Story in the Very Young versus the Very Old Romanian Patients. PLoS ONE 2015, 10, e0128010. [Google Scholar] [CrossRef]
- Ghiga, G.; Țarcă, E.; Țarcă, V.; Spoială, E.L.; Păduraru, G.; Gimiga, N.; Boca, L.O.; Iftinchi, O.; Donos, M.A.; Manole, L.M.; et al. Vitamin D Deficiency: Insights and Perspectives from a Five-Year Retrospective Analysis of Children from Northeastern Romania. Nutrients 2024, 16, 3808. [Google Scholar] [CrossRef]
- Herdea, A.; Marie, H.; Ionescu, A.; Sandu, D.-M.; Pribeagu, S.-T.; Ulici, A. Vitamin D Deficiency-A Public Health Issue in Children. Children 2024, 11, 1061. [Google Scholar] [CrossRef] [PubMed]
- Xi, L.; Wang, X.; Rao, J.; Zeng, Y.; Xiang, Y.; Li, C. High prevalence and seasonal patterns of vitamin D deficiency in children and adolescents in Central China: A three-year single-center study. Prev. Med. Rep. 2024, 48, 102929. [Google Scholar] [CrossRef] [PubMed]
- Feketea, G.M.; Bocsan, I.C.; Tsiros, G.; Voila, P.; Stanciu, L.A.; Zdrenghea, M. Vitamin D Status in Children in Greece and Its Relationship with Sunscreen Application. Children 2021, 8, 111. [Google Scholar] [CrossRef]
- Rahimi, B.A.; Khalid, A.A.; Usmani, A.; Khalid, W.A.; Baseer, A.Q.; Rahimi, J.A.; Taylor, W.R. Prevalence and risk factors of vitamin D deficiency among Afghan primary school children. Sci. Rep. 2024, 14, 27167. [Google Scholar] [CrossRef] [PubMed]
- Vierucci, F.; Del Pistoia, M.; Fanos, M.; Gori, M.; Carlone, G.; Erba, P.; Massimetti, G.; Federico, G.; Saggese, G. Vitamin D status and predictors of hypovitaminosis D in Italian children and adolescents: A cross-sectional study. Eur. J. Pediatr. 2013, 172, 1607–1617. [Google Scholar] [CrossRef] [PubMed]
- Van de Walle, L.; Vandenplas, Y.; Toelen, J.; Raaijmakers, A. Vitamin D Status in Belgian Children: A Regional Study. Nutrients 2024, 16, 657. [Google Scholar] [CrossRef]
- Manios, Y.; Moschonis, G.; Hulshof, T.; Bourhis, A.-S.; Hull, G.L.J.; Dowling, K.G.; Kiely, M.E.; Cashman, K.D. Prevalence of vitamin D deficiency and insufficiency among schoolchildren in Greece: The role of sex, degree of urbanisation and seasonality. Br. J. Nutr. 2017, 118, 550–558. [Google Scholar] [CrossRef]
- Nigg, C.; Weber, C.; Schipperijn, J.; Reichert, M.; Oriwol, D.; Worth, A.; Woll, A.; Niessner, C. Urban-Rural Differences in Children’s and Adolescent’s Physical Activity and Screen-Time Trends Across 15 Years. Health Educ. Behav. Off. Publ. Soc. Public Health Educ. 2022, 49, 789–800. [Google Scholar] [CrossRef]
- Peptine, L.D.; Răileanu, C.-R.; Goroftei, L.; Verga, G.-I.; Neagu, A.; Gurău, T.V.; Grigore, I.; Zaharia, A.-E.; Maftei, N.-M.; Matei, M.-N.; et al. The prevalence of vitamin D deficiency in a pediatric hospital in Romania. Innov. Romanian Food Biotechnol. 2023. [Google Scholar]
- Al-Taiar, A.; Rahman, A.; Al-Sabah, R.; Shaban, L.; Al-Harbi, A. Vitamin D status among adolescents in Kuwait: A cross-sectional study. BMJ Open 2018, 8, e021401. [Google Scholar] [CrossRef]
- Jayatissa, R.; Lekamwasam, S.; Ranbanda, J.M.; Ranasingha, S.; Perera, A.G.; De Silva, K.H. Vitamin D deficiency among children aged 10-18 years in Sri Lanka. Ceylon Med. J. 2019, 64, 146–154. [Google Scholar] [CrossRef] [PubMed]
- Niculescu, D.A.; Capatina, C.A.M.; Dusceac, R.; Caragheorgheopol, A.; Ghemigian, A.; Poiana, C. Seasonal variation of serum vitamin D levels in Romania. Arch. Osteoporos. 2017, 12, 113. [Google Scholar] [CrossRef] [PubMed]
- Grygorieva, N.V.; Solonenko, T.Y.; Musiienko, A.S.; Bystrytska, M.A. Vitamin D deficiency in Ukraine: Current evidence. BMC Nutr. 2023, 9, 49. [Google Scholar] [CrossRef] [PubMed]
- Delanghe, J.R.; Speeckaert, M.M.; Maenhout, T. Factors to take into account when interpreting 25-hydroxy-vitamin D serum levels. Acta Clin. Belg. 2024, 79, 97–102. [Google Scholar] [CrossRef]
- Osredkar, J.; Vičič, V.; Hribar, M.; Benedik, E.; Siuka, D.; Jerin, A.; Čegovnik Primožič, U.; Fabjan, T.; Kumer, K.; Pravst, I.; et al. Seasonal variation of total and bioavailable 25-hydroxyvitamin D [25(OH)D] in the healthy adult Slovenian population. Acta Biochim. Pol. 2024, 71, 13108. [Google Scholar] [CrossRef]
- Hribar, M.; Pravst, I.; Pogačnik, T.; Žmitek, K. Results of longitudinal Nutri-D study: Factors influencing winter and summer vitamin D status in a Caucasian population. Front. Nutr. 2023, 10, 1253341. [Google Scholar] [CrossRef]
- Kull, M.; Kallikorm, R.; Tamm, A.; Lember, M. Seasonal variance of 25-(OH) vitamin D in the general population of Estonia, a Northern European country. BMC Public Health 2009, 9, 22. [Google Scholar] [CrossRef]
- Davis, R.L.; Aksornsri, A.; Papachrisanthou, M.M. Vitamin D Screening Variations in Children and Adolescents: Who should be Screened? J. Pediatr. Nurs. 2019, 45, 57–61. [Google Scholar] [CrossRef]
- Pludowski, P.; Takacs, I.; Boyanov, M.; Belaya, Z.; Diaconu, C.C.; Mokhort, T.; Zherdova, N.; Rasa, I.; Payer, J.; Pilz, S. Clinical Practice in the Prevention, Diagnosis and Treatment of Vitamin D Deficiency: A Central and Eastern European Expert Consensus Statement. Nutrients 2022, 14, 1483. [Google Scholar] [CrossRef]
- Rockwell, M.; Kraak, V.; Hulver, M.; Epling, J. Clinical Management of Low Vitamin D: A Scoping Review of Physicians’ Practices. Nutrients 2018, 10, 493. [Google Scholar] [CrossRef]
- Pilz, S.; März, W.; Cashman, K.D.; Kiely, M.E.; Whiting, S.J.; Holick, M.F.; Grant, W.B.; Pludowski, P.; Hiligsmann, M.; Trummer, C.; et al. Rationale and Plan for Vitamin D Food Fortification: A Review and Guidance Paper. Front. Endocrinol. 2018, 9, 373. [Google Scholar] [CrossRef] [PubMed]
- Avdeeva, V.A.; Suplotova, L.A.; Pigarova, E.A.; Rozhinskaya, L.Y.; Troshina, E.A. Vitamin D deficiency in Russia: The first results of a registered, non-interventional study of the frequency of vitamin D deficiency and insufficiency in various geographic regions of the country. Probl. Endokrinol. 2021, 67, 84–92. [Google Scholar] [CrossRef] [PubMed]
- Cashman, K.D.; Dowling, K.G.; Škrabáková, Z.; Gonzalez-Gross, M.; Valtueña, J.; De Henauw, S.; Moreno, L.; Damsgaard, C.T.; Michaelsen, K.F.; Mølgaard, C.; et al. Vitamin D deficiency in Europe: Pandemic? Am. J. Clin. Nutr. 2016, 103, 1033–1044. [Google Scholar] [CrossRef] [PubMed]
- Lips, P.; Cashman, K.D.; Lamberg-Allardt, C.; Bischoff-Ferrari, H.A.; Obermayer-Pietsch, B.; Bianchi, M.L.; Stepan, J.; El-Hajj Fuleihan, G.; Bouillon, R. Current vitamin D status in European and Middle East countries and strategies to prevent vitamin D deficiency: A position statement of the European Calcified Tissue Society. Eur. J. Endocrinol. 2019, 180, P23–P54. [Google Scholar] [CrossRef]
- Nikolova, M.G.; Boyanov, M.A.; Tsakova, A.D. Correlations of Serum Vitamin D with Metabolic Parameters in Adult Outpatients with Different Degrees of Overweight/Obesity Coming from an Urban Community. Acta Endocrinol. Buchar. Rom. 2018, 14, 375–383. [Google Scholar] [CrossRef]
- Płudowski, P.; Ducki, C.; Konstantynowicz, J.; Jaworski, M. Vitamin D status in Poland. Pol. Arch. Med. Wewn. 2016, 126, 530–539. [Google Scholar] [CrossRef]
- Itkonen, S.T.; Andersen, R.; Björk, A.K.; Brugård Konde, Å.; Eneroth, H.; Erkkola, M.; Holvik, K.; Madar, A.A.; Meyer, H.E.; Tetens, I.; et al. Vitamin D status and current policies to achieve adequate vitamin D intake in the Nordic countries. Scand. J. Public Health 2021, 49, 616–627. [Google Scholar] [CrossRef]
- Wagner, C.L.; Greer, F.R.; American Academy of Pediatrics Section on Breastfeeding; American Academy of Pediatrics Committee on Nutrition. Prevention of rickets and vitamin D deficiency in infants, children, and adolescents. Pediatrics 2008, 122, 1142–1152. [Google Scholar] [CrossRef]
- Shakinba, M.; Tefagh, S.; Nafei, Z. The optimal dose of vitamin D in growing girls during academic years: A randomized trial. Turk. J. Med. Sci. 2011, 41, 33–37. [Google Scholar] [CrossRef]
- Kumaratne, M.; Vigneron, F.; Cisneros, J. Treatment with Vitamin D3 in Vitamin D Deficient Adolescents: A Pilot Study. Glob. Pediatr. Health 2020, 7, 2333794X20976240. [Google Scholar] [CrossRef]
- Durá-Travé, T.; Gallinas-Victoriano, F. COVID-19 in Children and Vitamin D. Int. J. Mol. Sci. 2024, 25, 12205. [Google Scholar] [CrossRef] [PubMed]
- Beyazgül, G.; Bağ, Ö.; Yurtseven, İ.; Coşkunol, F.; Başer, S.; Çiçek, D.; Kanberoğlu, G.İ.; Çelik, F.; Nalbantoğlu, Ö.; Özkan, B. How Vitamin D Levels of Children Changed During COVID-19 Pandemic: A Comparison of Pre-pandemic and Pandemic Periods. J. Clin. Res. Pediatr. Endocrinol. 2022, 14, 188–195. [Google Scholar] [CrossRef]
- Tomaszewska, A.; Rustecka, A.; Lipińska-Opałka, A.; Piprek, R.P.; Kloc, M.; Kalicki, B.; Kubiak, J.Z. The Role of Vitamin D in COVID-19 and the Impact of Pandemic Restrictions on Vitamin D Blood Content. Front. Pharmacol. 2022, 13, 836738. [Google Scholar] [CrossRef] [PubMed]
- Wang, X.; Cheng, Z. Cross-Sectional Studies: Strengths, Weaknesses, and Recommendations. Chest 2020, 158, S65–S71. [Google Scholar] [CrossRef] [PubMed]
Variable (Total) | Vitamin D Status | Ordinal Univariate Analysis | |||||
---|---|---|---|---|---|---|---|
Age Groups | Severe Deficiency (<12 ng/mL) (%) | Deficiency (12–20 ng/mL) (%) | Insufficiency (21–30 ng/mL) (%) | Sufficiency (>30 ng/mL) (%) | cOR | 95% CI | p-Value |
1–6 (893) | 8 (0.9) | 49 (5.49) | 186 (20.83) | 650 (72.79) | Ref. | ||
7–12 (356) | 20 (5.62) | 113 (31.74) | 159 (44.66) | 64 (17.98) | 0.17 | 0.13–0.21 | <0.001 |
13–18 (449) | 4 (0.89) | 85 (18.93%) | 236 (52.56) | 124 (27.62) | 0.08 | 0.06–0.11 | <0.001 |
Area of residence | |||||||
Rural (389) | 6 (1.54) | 44 (11.31) | 131(33.68) | 208 (53.47) | Ref. | ||
Urban (1309) | 26 (1.99) | 203 (15.51) | 450 (34.38) | 630 (48.13) | 0.78 | 0.63–0.97 | 0.03 |
Gender | |||||||
Female (812) | 18 (2.22) | 130 (16.01) | 283 (34.85) | 381 (46.92) | Ref. | ||
Male (886) | 14 (1.58) | 117 (13.21) | 298 (33.63) | 457 (51.58) | 1.23 | 1.02–1.47 | 0.03 |
Total (1698) | 32 (1.88) | 247 (14.55) | 581 (34.22) | 838 (49.35%) |
Variable (Total) | Vitamin D Status | Univariate Analysis | |||||
---|---|---|---|---|---|---|---|
Month | Severe Deficiency (<12 ng/mL) (%) | Deficiency (12–20 ng/mL) (%) | Insufficiency (21–30 ng/mL) (%) | Sufficiency (>30 ng/mL) (%) | cOR | 95% CI | p -Value |
January (147) | 9 (6.12) | 22 (14.97) | 57 (38.78) | 59 (40.14) | Ref. | ||
February (171) | 3 (1.75) | 30 (17.54) | 68 (39.77) | 70 (40.94) | 1.09 | 0.72–1.65 | 0.67 |
March (179) | 7 (3.91) | 45 (25.14) | 70 (39.11) | 57 (31.84) | 0.69 | 0.46–1.04 | 0.08 |
April (131) | 2 (1.53) | 28 (21.37) | 51 (38.93) | 50 (38.17) | 0.95 | 0.61–1.47 | 0.81 |
May (182) | 2 (1.1) | 32 (17.58) | 68 (37.36) | 80 (43.96) | 1.21 | 0.80–1.82 | 0.36 |
June (159) | 3 (1.89) | 29 (18.24) | 45 (28.3) | 82 (51.57) | 1.47 | 0.96–2.26 | 0.08 |
July (134) | 4 (2.99) | 12 (8.96) | 38 (28.36) | 80 (59.7) | 2.22 | 1.41–3.49 | 0.001 |
August (113) | 0 (0) | 5 (4.42) | 33 (29.2) | 75 (66.37) | 3.21 | 1.97–5.23 | <0.001 |
September (123) | 0 (0) | 8 (6.5) | 29 (23.58) | 86 (69.92) | 3.61 | 2.22–5.88 | <0.001 |
October (126) | 1 (0.79) | 10 (7.94) | 33 (26.19) | 82 (65.08) | 2.87 | 1.79–4.59 | <0.001 |
November (126) | 0 (0) | 13 (10.32) | 55 (43.65) | 58 (46.03) | 1.5 | 0.96–2.33 | 0.08 |
December (107) | 1 (0.93) | 13 (12.15) | 34 (31.78) | 59 (55.14) | 1.88 | 1.17–3.03 | 0.01 |
Total (1698) | 32 (1.88%) | 247 (14.55) | 581 (34.22) | 838 (49.35) |
Variable (Total) | Vitamin D Status | Ordinal Univariate Analysis | |||||
---|---|---|---|---|---|---|---|
Year | Severe Deficiency (<12 ng/mL) (%) | Deficiency (12–20 ng/mL) (%) | Insufficiency (21–30 ng/mL) (%) | Sufficiency (>30 ng/mL) (%) | cOR | 95% CI | p-Value |
2018 (369) | 4 (1.08) | 61 (16.53) | 131 (35.5) | 173 (46.88) | Ref. | ||
2019 (528) | 9 (1.7) | 70 (13.26) | 179 (33.9) | 270 (51.14) | 1.19 | 0.92–1.52 | 0.19 |
2020 (473) | 11 (2.33) | 51 (10.78) | 156 (32.98) | 255 (53.91) | 1.33 | 1.03–1.72 | 0.03 |
2021 (328) | 8 (2.44) | 65 (19.82) | 115 (35.06) | 140 (42.68) | 0.81 | 0.61–1.07 | 0.13 |
Total (1698) | 32 (1.88) | 247 (14.55) | 581 (34.22) | 838 (49.35) |
Multivariate Analysis | |||
---|---|---|---|
Variable | aOR | 95% CI | p-Value |
Age groups | |||
7–12 | 0.16 | 0.13–0.21 | <0.001 |
13–18 | 0.07 | 0.05–0.09 | <0.001 |
Year | |||
2019 | 1.49 | 1.14–1.97 | 0.004 |
2020 | 1.73 | 1.29–2.32 | <0.001 |
2021 | 1.63 | 1.19–2.24 | 0.003 |
Month | |||
July | 2.61 | 1.58–4.30 | <0.001 |
August | 3.71 | 2.16–6.38 | <0.001 |
September | 5.47 | 3.17–9.42 | <0.001 |
October | 4.52 | 2.68–7.64 | <0.001 |
November | 1.83 | 1.13–2.96 | 0.013 |
December | 2.2 | 1.31–3.71 | 0.003 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2025 by the authors. Published by MDPI on behalf of the Lithuanian University of Health Sciences. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Mihu, A.G.; Nicolescu, C.M.; Marc, C.C.; Boru, C.; Susan, M.; Ciceu, A.; Sprintar, S.A.; Olariu, A.T.; Oatis, D.A.; Nicolescu, L.C.; et al. Retrospective Serologic Assessment of Vitamin D Levels in Children from Western Romania: A Cross-Sectional Study. Medicina 2025, 61, 394. https://doi.org/10.3390/medicina61030394
Mihu AG, Nicolescu CM, Marc CC, Boru C, Susan M, Ciceu A, Sprintar SA, Olariu AT, Oatis DA, Nicolescu LC, et al. Retrospective Serologic Assessment of Vitamin D Levels in Children from Western Romania: A Cross-Sectional Study. Medicina. 2025; 61(3):394. https://doi.org/10.3390/medicina61030394
Chicago/Turabian StyleMihu, Alin Gabriel, Cristian Mircea Nicolescu, Constantin Catalin Marc, Casiana Boru, Monica Susan, Alina Ciceu, Sergiu Adrian Sprintar, Alexander Tudor Olariu, Daniela Adriana Oatis, Laura Corina Nicolescu, and et al. 2025. "Retrospective Serologic Assessment of Vitamin D Levels in Children from Western Romania: A Cross-Sectional Study" Medicina 61, no. 3: 394. https://doi.org/10.3390/medicina61030394
APA StyleMihu, A. G., Nicolescu, C. M., Marc, C. C., Boru, C., Susan, M., Ciceu, A., Sprintar, S. A., Olariu, A. T., Oatis, D. A., Nicolescu, L. C., & Olariu, T. R. (2025). Retrospective Serologic Assessment of Vitamin D Levels in Children from Western Romania: A Cross-Sectional Study. Medicina, 61(3), 394. https://doi.org/10.3390/medicina61030394