Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against Serotypes C and K of Streptococcus mutans on Orthodontic Appliances
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis and Characterization of AgNP
2.2. Identification of S. mutans Serotypes by Polymerase Chain Reaction (PCR)
2.3. S. mutans Suspensions and Antibacterial Test
2.4. Preparation of Dental Samples
2.5. Adherence Testing
2.6. SEM and AFM Analysis
2.7. Statistical Analysis
3. Results
3.1. Characterization of AgNP
3.2. Adherence Testing
3.3. SEM and AFM Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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AgNP (nm) | DLS Diameter (nm) | Shape | Initial Concentration (µg/mL) | Zeta Potential (mV) | MIC in Serotype c (µg/mL) | MIC in Serotype k (µg/mL) |
---|---|---|---|---|---|---|
29.3 | 29.3 ± 0.7 | Spherical | 1070 | −36.5 ± 5.7 | 55.7 ± 19.3 | 111.4 ± 38.6 |
AgNP 29.3 nm (CFU/mL × 104) | Control (CFU/mL × 104) | Total (CFU/mL × 104) | |
---|---|---|---|
Serotype | |||
c | 0.5 ± 0.7 * | 87.5 ± 80.9 | 44.0 ± 71.4 |
k | 4.2 ± 7.7 * | 243.5 ± 216.1 | 123.9 ± 193.1 |
Archwire | |||
N/A | 0.3 ± 0.8 * | 164.5 ± 19.2 a | 82.4 ± 86.7 |
SS | 8.6 ± 9.1 | 289.3 ± 306.8 | 149.0 ± 253.6 a |
CuNiTi | 0.1 ± 0.4 | 6.5 ± 7.5 a,b | 3.3 ± 6.0 a |
NiTi | 0.3 ± 0.8 * | 202.0 ± 38.7 b | 101.1 ± 108.5 |
Total | 2.3 ± 5.6 * | 165.5 ± 178.4 | - |
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Nafarrate-Valdez, R.A.; Martínez-Martínez, R.E.; Zaragoza-Contreras, E.A.; Áyala-Herrera, J.L.; Domínguez-Pérez, R.A.; Reyes-López, S.Y.; Donohue-Cornejo, A.; Cuevas-González, J.C.; Loyola-Rodríguez, J.P.; Espinosa-Cristóbal, L.F. Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against Serotypes C and K of Streptococcus mutans on Orthodontic Appliances. Medicina 2022, 58, 877. https://doi.org/10.3390/medicina58070877
Nafarrate-Valdez RA, Martínez-Martínez RE, Zaragoza-Contreras EA, Áyala-Herrera JL, Domínguez-Pérez RA, Reyes-López SY, Donohue-Cornejo A, Cuevas-González JC, Loyola-Rodríguez JP, Espinosa-Cristóbal LF. Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against Serotypes C and K of Streptococcus mutans on Orthodontic Appliances. Medicina. 2022; 58(7):877. https://doi.org/10.3390/medicina58070877
Chicago/Turabian StyleNafarrate-Valdez, Rosa Amalia, Rita Elizabeth Martínez-Martínez, Erasto Armando Zaragoza-Contreras, José Luis Áyala-Herrera, Rubén Abraham Domínguez-Pérez, Simón Yobanny Reyes-López, Alejandro Donohue-Cornejo, Juan Carlos Cuevas-González, Juan Pablo Loyola-Rodríguez, and León Francisco Espinosa-Cristóbal. 2022. "Anti-Adherence and Antimicrobial Activities of Silver Nanoparticles against Serotypes C and K of Streptococcus mutans on Orthodontic Appliances" Medicina 58, no. 7: 877. https://doi.org/10.3390/medicina58070877