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Volume 47
Issue 12
10.3390/cimb47121005
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

In Silico and Wet Analysis of BAX Gene G-248A Polymorphism and mRNA Expression in Peptic Ulcer Disease and Gastric Cancer

by
Marta Żebrowska-Nawrocka
1,
Rafał Świechowski
1,*,
Dagmara Szmajda-Krygier
1,
Bartosz Lenda
1,
Marek Mirowski
1,*,
Michał Czaplij
2,
Mariusz Balcerczak
2 and
Ewa Balcerczak
1
1
Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, 90-151 Lodz, Poland
2
Department of Surgery, District Hospital in Leczyca,99-100 Leczyca, Poland
*
Authors to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2025, 47(12), 1005; https://doi.org/10.3390/cimb47121005 (registering DOI)
Submission received: 6 November 2025 / Revised: 27 November 2025 / Accepted: 28 November 2025 / Published: 29 November 2025
(This article belongs to the Section Molecular Medicine)
Versions Notes

Abstract

Peptic ulcer disease and gastric cancer are influenced by both environmental factors and genetic background. One such genetic factor is changes in the BAX gene, where G-248A decreases the activity of the BAX gene promoter, thereby inhibiting apoptosis and promoting carcinogenesis. The relationship between the BAX gene and the risk of developing these diseases has not been fully elucidated. In this study, genotyping G-248A was performed by restriction fragment-length polymorphism, and real-time PCR was employed to quantify BAX mRNA expression. An in silico analysis was performed using publicly available databases. The findings reveal a significant prevalence of the AA genotype in the gastric cancer group compared to healthy individuals, suggesting a potential genetic predisposition to malignancy. When peptic ulcer group and healthy controls were compared, no significant association was found. Further, in silico analyses demonstrated elevated BAX expression in gastric cancer tissues, correlating with advanced histological grades and improved overall survival rates. Elevated BAX expression, however, is associated with gastric cancer onset and could be a promising prognostic indicator. This study underlines the complex correlation between genetic factors and disease, highlighting the potential of the BAX gene as a biomarker for gastric cancer prognosis and therapeutic targeting.
Keywords: carcinogenesis; apoptosis; expression; polymorphisms; BAX; SNP G-248A; rs4645878 carcinogenesis; apoptosis; expression; polymorphisms; BAX; SNP G-248A; rs4645878

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MDPI and ACS Style

Żebrowska-Nawrocka, M.; Świechowski, R.; Szmajda-Krygier, D.; Lenda, B.; Mirowski, M.; Czaplij, M.; Balcerczak, M.; Balcerczak, E. In Silico and Wet Analysis of BAX Gene G-248A Polymorphism and mRNA Expression in Peptic Ulcer Disease and Gastric Cancer. Curr. Issues Mol. Biol. 2025, 47, 1005. https://doi.org/10.3390/cimb47121005

AMA Style

Żebrowska-Nawrocka M, Świechowski R, Szmajda-Krygier D, Lenda B, Mirowski M, Czaplij M, Balcerczak M, Balcerczak E. In Silico and Wet Analysis of BAX Gene G-248A Polymorphism and mRNA Expression in Peptic Ulcer Disease and Gastric Cancer. Current Issues in Molecular Biology. 2025; 47(12):1005. https://doi.org/10.3390/cimb47121005

Chicago/Turabian Style

Żebrowska-Nawrocka, Marta, Rafał Świechowski, Dagmara Szmajda-Krygier, Bartosz Lenda, Marek Mirowski, Michał Czaplij, Mariusz Balcerczak, and Ewa Balcerczak. 2025. "In Silico and Wet Analysis of BAX Gene G-248A Polymorphism and mRNA Expression in Peptic Ulcer Disease and Gastric Cancer" Current Issues in Molecular Biology 47, no. 12: 1005. https://doi.org/10.3390/cimb47121005

APA Style

Żebrowska-Nawrocka, M., Świechowski, R., Szmajda-Krygier, D., Lenda, B., Mirowski, M., Czaplij, M., Balcerczak, M., & Balcerczak, E. (2025). In Silico and Wet Analysis of BAX Gene G-248A Polymorphism and mRNA Expression in Peptic Ulcer Disease and Gastric Cancer. Current Issues in Molecular Biology, 47(12), 1005. https://doi.org/10.3390/cimb47121005

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