Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Development in Maize
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of ZmFAR1 Genes in Maize B73
2.2. Phylogenetic Analyses of ZmFAR1 Genes
2.3. Gene Structure and Conserved Motif Analysis of ZmFAR1 Genes
2.4. Chromosomal Location and Collinearity Analysis of ZmFAR1 Genes
2.5. Cis-Acting Elements and Interaction Network Analysis of ZmFAR1 Genes
2.6. Tissue-Specific Expression Analysis of ZmFAR1 Genes
2.7. Plant Materials and Stress Treatments
2.8. RNA Isolation and Quantitative qRT-PCR Analysis
2.9. Expression Quantitative Trait Loci (eQTL) Analysis
2.10. Statistical Analysis
3. Results
3.1. Identification and Chromosomal Location of ZmFAR1 Genes in Maize
3.2. Phylogenetic Analysis of ZmFAR1 Genes
3.3. Conserved Structure and Motif Analyses of ZmFAR1 Genes
3.4. Collinearity Analysis of ZmFAR1 Genes
3.5. Cis-Acting Element Analysis of ZmFAR1 Genes
3.6. Target miRNA Prediction and Interactive Network Analysis of ZmFAR1 Genes
3.7. Expression Analysis of ZmFAR1 Genes
3.8. Expression Analysis of ZmFAR1 Genes under Far-Red Light
3.9. Cis-eQTL Analyses Identify the Causal Variations Associated with Gene Expression Variation and Agronomic Traits
4. Discussion
4.1. Characterization of the ZmFAR1 Family Members in Maize
4.2. Studies on the Structure, Evolutionary Characteristics, and Functions of Maize ZmFAR1 Genes
4.3. Functional Variations and Expression Patterns of ZmFAR1 Genes in Maize Ear Development and Phenotypic Variation
4.4. ZmFAR1 Genes’ Role in Maize Yield and Trait Optimization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Tang, H.; Jing, D.; Liu, C.; Xie, X.; Zhang, L.; Chen, X.; Li, C. Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Development in Maize. Curr. Issues Mol. Biol. 2024, 46, 430-449. https://doi.org/10.3390/cimb46010027
Tang H, Jing D, Liu C, Xie X, Zhang L, Chen X, Li C. Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Development in Maize. Current Issues in Molecular Biology. 2024; 46(1):430-449. https://doi.org/10.3390/cimb46010027
Chicago/Turabian StyleTang, Huaijun, De Jing, Cheng Liu, Xiaoqing Xie, Lei Zhang, Xunji Chen, and Changyu Li. 2024. "Genome-Wide Identification and Expression Analyses of the FAR1/FHY3 Gene Family Provide Insight into Inflorescence Development in Maize" Current Issues in Molecular Biology 46, no. 1: 430-449. https://doi.org/10.3390/cimb46010027