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Article

Effect of Metformin and Simvastatin in Inhibiting Proadipogenic Transcription Factors

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Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Josipa Huttlera, 4, 31000 Osijek, Croatia
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Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Osijek, Crkvena 21, 31000 Osijek, Croatia
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Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21137 Novi Sad, Serbia
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Polyclinic Ćosić, Petra Preradovića 4, 35000 Slavonski Brod, Croatia
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Zadar General Hospital, Ul. Bože Peričića 5, 23000 Zadar, Croatia
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General Hospital “Dr. Josip Benčević” Slavonski Brod, Ul. Andrije Štampara 42, 35000 Slavonski Brod, Croatia
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Author to whom correspondence should be addressed.
Academic Editor: Hidayat Hussain
Curr. Issues Mol. Biol. 2021, 43(3), 2082-2097; https://doi.org/10.3390/cimb43030144 (registering DOI)
Received: 27 October 2021 / Revised: 13 November 2021 / Accepted: 18 November 2021 / Published: 25 November 2021
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Obesity is a multifactorial chronic disease characterized by the excessive accumulation of fat in adipose tissue driven by hypertrophy and hyperplasia of adipocytes through adipogenesis. Adipogenesis plays a key role in the development of obesity and related metabolic disorders, which makes it potential target for the therapeutic approach to obesity. An increasing number of studies confirm the pleiotropic action of the combined treatment with metformin and statins, suggesting their anti-hypertensive, anti-inflammatory, and anti-adipogenic effect. The aim of this study was to analyze the effect of different doses of metformin (MET) and simvastatin (SIM) on the expression of key transcription factors of adipogenesis. Mouse 3T3-L1 preadipocytes were induced to differentiation in adipogenic medium with sustained MET and SIM treatment to assess the effect on adipogenesis. Nine days after initiating adipogenesis, the cells were prepared for further experiments, including Oil Red O staining, RT-PCR, Western blotting, and immunocytochemistry. Treating the cells with the combination of MET and SIM slightly reduced the intensity of Oil Red O staining compared with the control group, and down-regulated mRNA and protein expression of PPARγ, C/EBPα, and SREBP-1C. In conclusion, the inhibitory effect of MET and SIM on adipocyte differentiation, as indicated by decreased lipid accumulation, appears to be mediated through the down-regulation of adipogenic transcription factors, peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding pro-tein α (C/EBPα), and sterol regulatory element-binding protein 1 (SREBP-1C). View Full-Text
Keywords: adipocytes; adipogenesis; obesity; metformin; simvastatin adipocytes; adipogenesis; obesity; metformin; simvastatin
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MDPI and ACS Style

Jakab, J.; Zjalić, M.; Mikšić, Š.; Tušek, I.; Ćosić, V.; Volarić, N.; Nakić, D.; Včev, A.; Miškić, B. Effect of Metformin and Simvastatin in Inhibiting Proadipogenic Transcription Factors. Curr. Issues Mol. Biol. 2021, 43, 2082-2097. https://doi.org/10.3390/cimb43030144

AMA Style

Jakab J, Zjalić M, Mikšić Š, Tušek I, Ćosić V, Volarić N, Nakić D, Včev A, Miškić B. Effect of Metformin and Simvastatin in Inhibiting Proadipogenic Transcription Factors. Current Issues in Molecular Biology. 2021; 43(3):2082-2097. https://doi.org/10.3390/cimb43030144

Chicago/Turabian Style

Jakab, Jelena, Milorad Zjalić, Štefica Mikšić, Ivan Tušek, Vesna Ćosić, Nikola Volarić, Dario Nakić, Aleksandar Včev, and Blaženka Miškić. 2021. "Effect of Metformin and Simvastatin in Inhibiting Proadipogenic Transcription Factors" Current Issues in Molecular Biology 43, no. 3: 2082-2097. https://doi.org/10.3390/cimb43030144

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