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Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System

1
OrthoSera GmbH, 3500 Krems, Austria
2
Center for Regenerative Medicine, Danube University Krems, 3500 Krems, Austria
3
Institute of Sport and Health Sciences, University of Physical Education, H-1123 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Luca Testarelli
Curr. Issues Mol. Biol. 2021, 43(2), 665-675; https://doi.org/10.3390/cimb43020048
Received: 9 June 2021 / Revised: 6 July 2021 / Accepted: 8 July 2021 / Published: 11 July 2021
(This article belongs to the Section Molecular Medicine)
Intra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. Chondrocyte viability was determined by XTT assay and it revealed no significant difference in metabolic activity of OA chondrocyte pellets after supplementation with different blood products. Nevertheless, the selection of blood products influenced the cartilage-related genes expression, ECM morphology and the tissue quality of pellets. Both PRP types had a different biological effect depending upon concentration and even though CPRP is widely used in clinics our assessment did not reveal good results in gene expression either tissue quality. HypACT supplementation resulted in superior cartilage-related genes expression together with tissue quality and seemed to be the most stable product since no remarkable changes were observed between the two different concentrations. All in all, for successful regenerative therapy, possible molecular mechanisms induced by blood-derived products should be always carefully investigated and adapted to the specific medical indications. View Full-Text
Keywords: osteoarthritic chondrocytes; cartilage; 3D pellet culture; blood products; platelet-rich plasma; hyperacute serum osteoarthritic chondrocytes; cartilage; 3D pellet culture; blood products; platelet-rich plasma; hyperacute serum
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MDPI and ACS Style

Kuten-Pella, O.; De Luna, A.; Kramer, K.; Neubauer, M.; Nehrer, S.; Lacza, Z. Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System. Curr. Issues Mol. Biol. 2021, 43, 665-675. https://doi.org/10.3390/cimb43020048

AMA Style

Kuten-Pella O, De Luna A, Kramer K, Neubauer M, Nehrer S, Lacza Z. Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System. Current Issues in Molecular Biology. 2021; 43(2):665-675. https://doi.org/10.3390/cimb43020048

Chicago/Turabian Style

Kuten-Pella, Olga, Andrea De Luna, Karina Kramer, Markus Neubauer, Stefan Nehrer, and Zsombor Lacza. 2021. "Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System" Current Issues in Molecular Biology 43, no. 2: 665-675. https://doi.org/10.3390/cimb43020048

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