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Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening

1
Institut de Génomique Fonctionnelle, CNRS, UMR 5203, Montpellier, France and INSERM, U. 661, Montpellier, France and Université Montpellier,1,2, Montpellier, France
2
CIS bio international, BP 84175, F-30204 Bagnols sur Cèze Cedex, France
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2011, 4(1), 202-214; https://doi.org/10.3390/ph4010202
Received: 1 December 2010 / Revised: 23 December 2010 / Accepted: 24 December 2010 / Published: 18 January 2011
(This article belongs to the Special Issue GPCR Based Drug Discovery)
The identification of new drugs exhibiting reduced adverse side-effects constitutes a great challenge for the next decade. Various steps are needed to screen for good ligand candidates and one of them is the evaluation of their binding properties. New strategies based on fluorescence measurement constitute excellent alternatives to the traditional radioactive assays. Less hazardous, faster and cheaper, these methods also exhibit very good sensitivity and can be used on various biological models such as heterologous expression systems or native tissues. View Full-Text
Keywords: screening assay; fluorescence; lanthanides; time resolved FRET; GPCR screening assay; fluorescence; lanthanides; time resolved FRET; GPCR
MDPI and ACS Style

Cottet, M.; Faklaris, O.; Zwier, J.M.; Trinquet, E.; Pin, J.-P.; Durroux, T. Original Fluorescent Ligand-Based Assays Open New Perspectives in G-Protein Coupled Receptor Drug Screening. Pharmaceuticals 2011, 4, 202-214.

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