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Article

Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines

1
Institute of Molecular Biology “ Acad. Roumen Tsanev”, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bld. 21, 1113 Sofia, Bulgaria
2
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bld. 9, 1113 Sofia, Bulgaria
*
Authors to whom correspondence should be addressed.
Pharmaceuticals 2023, 16(2), 181; https://doi.org/10.3390/ph16020181
Received: 6 December 2022 / Revised: 16 January 2023 / Accepted: 18 January 2023 / Published: 24 January 2023

Abstract

This study is the first report describing the promising antitumor activity of biologically active compounds isolated from the hemolymph of marine snail Rapana venosa—a fraction with Mw between 50 and 100 kDa and two structural subunits (RvH1 and RvH2), tested on a panel of human breast cell lines—six lines of different molecular subtypes of breast cancer MDA-MB-231, MDA-MB-468, BT-474, BT-549, SK-BR-3, and MCF-7 and the non-cancerous MCF-10A. The fraction with Mw 50–100 kDa (HRv 50–100) showed good antitumor activity manifested by a significant decrease in cell viability, altered morphology, autophagy, and p53 activation in treated cancer cells. An apparent synergistic effect was observed for the combination of HRv 50–100 with cis-platin for all tested cell lines. The combination of HRv 50–100 with cisplatin and/or tamoxifen is three times more effective compared to treatment with classical chemotherapeutics alone. The main proteins in the active fraction, with Mw at ~50 kDa, ~65 kDa, ~100 kDa, were identified by MALDI-MS, MS/MS analyses, and bioinformatics. Homology was established with known proteins with antitumor potential detected in different mollusc species: peroxidase-like protein, glycoproteins Aplysianin A, L-amino acid oxidase (LAAO), and the functional unit with Mw 50 kDa of RvH. Our study reveals new perspectives for application of HRv 50–100 as an antitumor agent used alone or as a booster in combination with different chemotherapies.
Keywords: Rapana venosa hemolymph; breast cancer; cisplatin; tamoxifen; bioactive compounds; mass spectrometry Rapana venosa hemolymph; breast cancer; cisplatin; tamoxifen; bioactive compounds; mass spectrometry

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MDPI and ACS Style

Petrova, M.; Vlahova, Z.; Schröder, M.; Todorova, J.; Tzintzarov, A.; Gospodinov, A.; Velkova, L.; Kaynarov, D.; Dolashki, A.; Dolashka, P.; Ugrinova, I. Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines. Pharmaceuticals 2023, 16, 181. https://doi.org/10.3390/ph16020181

AMA Style

Petrova M, Vlahova Z, Schröder M, Todorova J, Tzintzarov A, Gospodinov A, Velkova L, Kaynarov D, Dolashki A, Dolashka P, Ugrinova I. Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines. Pharmaceuticals. 2023; 16(2):181. https://doi.org/10.3390/ph16020181

Chicago/Turabian Style

Petrova, Maria, Zlatina Vlahova, Maria Schröder, Jordana Todorova, Alexander Tzintzarov, Anastas Gospodinov, Lyudmila Velkova, Dimitar Kaynarov, Aleksandar Dolashki, Pavlina Dolashka, and Iva Ugrinova. 2023. "Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines" Pharmaceuticals 16, no. 2: 181. https://doi.org/10.3390/ph16020181

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