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Article

MgAl and ZnAl-Hydrotalcites as Materials for Cosmetic and Pharmaceutical Formulations: Study of Their Cytotoxicity on Different Cell Lines

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Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy
2
Department of Physics and Geology, University of Perugia, 06123 Perugia, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Dimitris Tsiourvas
Pharmaceuticals 2022, 15(7), 784; https://doi.org/10.3390/ph15070784 (registering DOI)
Received: 3 June 2022 / Revised: 20 June 2022 / Accepted: 21 June 2022 / Published: 23 June 2022
(This article belongs to the Section Pharmaceutical Technology)
The knowledge about the effect of hydrotalcites (HTlcs), largely used in pharmaceutics, on non-malignant cell lines is limited. The effect of MgAl-HTlc-and ZnAl-HTlc- (NO3−/Cl/CO32−) on the cell viability of HaCat, fibroblasts and HepG2 was studied by MTT assay. Cells were incubated either with HTlc suspensions in the culture media and with the supernatant obtained from the suspension being centrifuged. MgAl-HTlcs suspensions resulted in being cytotoxic. As SEM and TEM analyses showed the presence of sub-micrometric particles in all the MgAl-HTlc examined, it could be hypothesized that this fraction can be internalized into cells reducing the viability. MgAl-HTlc-NO3 is the most cytotoxic probably due to the additional effect of NO3 anions. ZnAl-HTlcs are cytotoxic, especially for HaCat and HepG2 cells (viability <60% at all the concentrations assayed). The effect is attributable both to the sub-micrometric fraction (identified by TEM) and to the high Zn2+ levels found in the culture medium by ICP-OES analysis, suggesting that ZnAl-HTlcs are less stable than MgAl-HTlc in the used media. The obtained results suggest that it is very important to perform ad hoc studies in order to evaluate HTlc safety before to be introduced in a formulation. View Full-Text
Keywords: hydrotalcites; layered double hydroxides; MgAl-HTlc; ZnAl-HTlc; HaCat; fibroblasts; HepG2; cytotoxicity hydrotalcites; layered double hydroxides; MgAl-HTlc; ZnAl-HTlc; HaCat; fibroblasts; HepG2; cytotoxicity
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MDPI and ACS Style

Ceccarini, M.R.; Puccetti, M.; Pagano, C.; Nocchetti, M.; Beccari, T.; di Michele, A.; Ricci, M.; Perioli, L. MgAl and ZnAl-Hydrotalcites as Materials for Cosmetic and Pharmaceutical Formulations: Study of Their Cytotoxicity on Different Cell Lines. Pharmaceuticals 2022, 15, 784. https://doi.org/10.3390/ph15070784

AMA Style

Ceccarini MR, Puccetti M, Pagano C, Nocchetti M, Beccari T, di Michele A, Ricci M, Perioli L. MgAl and ZnAl-Hydrotalcites as Materials for Cosmetic and Pharmaceutical Formulations: Study of Their Cytotoxicity on Different Cell Lines. Pharmaceuticals. 2022; 15(7):784. https://doi.org/10.3390/ph15070784

Chicago/Turabian Style

Ceccarini, Maria R., Matteo Puccetti, Cinzia Pagano, Morena Nocchetti, Tommaso Beccari, Alessandro di Michele, Maurizio Ricci, and Luana Perioli. 2022. "MgAl and ZnAl-Hydrotalcites as Materials for Cosmetic and Pharmaceutical Formulations: Study of Their Cytotoxicity on Different Cell Lines" Pharmaceuticals 15, no. 7: 784. https://doi.org/10.3390/ph15070784

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