Effects of Fisetin, a Plant-Derived Flavonoid, on Response to Oxidative Stress, Aging, and Age-Related Diseases in Caenorhabditis elegans
Abstract
:1. Introduction
2. Results
2.1. Fisetin Shows Radical-Scavenging Activity and Reduces Cellular ROS Levels
2.2. Fisetin Increases Resistance to Oxidative Stress
2.3. Fisetin Extends Lifespan of C. elegans and Reduces Fertility
2.4. Age-Related Decline of Locomotive Activity Is Delayed by Fisetin
2.5. Fisetin Delays Paralysis Induced by Aβ, Which Requires DAF-16 and SKN-1
2.6. Reduced Survival by High-Glucose Diet (HGD) Is Recovered by Fisetin through SKN-1
2.7. Fisetin Prevents Degeneration of Dopaminergic Neurons
2.8. DAF-16-Induced Response and Autophagy Are Involved in Lifespan Extension by Fisetin
3. Discussion
4. Materials and Methods
4.1. Worm Strains and Culture
4.2. Radical Scavenging Assay
4.3. ROS Measurement
4.4. Responses to Environmental Stresses
4.5. Lifespan Assay
4.6. Fertility Assay
4.7. Measurement of Locomotive Activity
4.8. Paralysis Induced by Human Aβ Transgene
4.9. Survival under HGD
4.10. Degeneration of Dopaminergic Neurons
4.11. RNAi with Bacterial Feeding
4.12. Quantitative RT-PCR
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, S.; Kim, B.-K.; Park, S.-K. Effects of Fisetin, a Plant-Derived Flavonoid, on Response to Oxidative Stress, Aging, and Age-Related Diseases in Caenorhabditis elegans. Pharmaceuticals 2022, 15, 1528. https://doi.org/10.3390/ph15121528
Park S, Kim B-K, Park S-K. Effects of Fisetin, a Plant-Derived Flavonoid, on Response to Oxidative Stress, Aging, and Age-Related Diseases in Caenorhabditis elegans. Pharmaceuticals. 2022; 15(12):1528. https://doi.org/10.3390/ph15121528
Chicago/Turabian StylePark, Suhyeon, Bo-Kyoung Kim, and Sang-Kyu Park. 2022. "Effects of Fisetin, a Plant-Derived Flavonoid, on Response to Oxidative Stress, Aging, and Age-Related Diseases in Caenorhabditis elegans" Pharmaceuticals 15, no. 12: 1528. https://doi.org/10.3390/ph15121528