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Photoprotective Effects of Selected Amino Acids on Naproxen Photodegradation in Aqueous Media

1
Faculty of Pharmacy, Yasuda Women’s University, 6-13-1 Yasuhigashi, Asaminami-ku, Hiroshima 731-0153, Japan
2
Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(6), 135; https://doi.org/10.3390/ph13060135
Received: 28 May 2020 / Revised: 11 June 2020 / Accepted: 25 June 2020 / Published: 26 June 2020
It is important to develop a photostabilization strategy to ensure the quality of photosensitive compounds, including pharmaceuticals. This study focused on the protective effects of 20 amino acids on the photodegradation of naproxen (NX), a photosensitive pharmaceutical, to clarify the important nature of a good photostabilizer. Our previous report indicated the photodegradability of NX and the protective effects of some antioxidants on its photodegradation, therefore, this compound was used as a model compound. The degradation of NX in aqueous media during ultraviolet light (UV) irradiation and the protective effects of selected amino acids were monitored through high-performance liquid chromatography (HPLC), equipped with a reverse-phase column. Addition of cysteine, tryptophan, and tyrosine induced the significant suppression of NX photodegradation after UV irradiation for 3 h (residual amount of NX; 15.35%, 6.82%, and 15.64%, respectively). Evaluation of the antioxidative activity and UV absorption spectrum showed that cysteine suppressed NX degradation through its antioxidative ability, while tryptophan and tyrosine suppressed it through their UV filtering ability. Furthermore, three amino acids at higher concentrations (more than 100 µmol/L) showed more protective effects on NX photodegradation. For 10 mmol/L, residual amounts of NX with cysteine, tryptophan, and tyrosine were 58.51%, 69.34%, and 82.40%, respectively. These results showed the importance of both photoprotective potencies (antioxidative potency and UV filtering potency) and stability to UV irradiation for a good photostabilizer of photosensitive pharmaceuticals. View Full-Text
Keywords: analytical chemistry; antioxidative activity; chromatography; HPLC; photochemistry; photodegradation; photostability; photostabilization; UV/Vis spectroscopy analytical chemistry; antioxidative activity; chromatography; HPLC; photochemistry; photodegradation; photostability; photostabilization; UV/Vis spectroscopy
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Kawabata, K.; Kanoh, M.; Okazaki, M.; Maeda, R.; Mori, S.; Akimoto, S.; Inagaki, M.; Nishi, H. Photoprotective Effects of Selected Amino Acids on Naproxen Photodegradation in Aqueous Media. Pharmaceuticals 2020, 13, 135.

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