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Open AccessArticle

Micromeria fruticosa Induces Cell Cycle Arrest and Apoptosis in Breast and Colorectal Cancer Cells

1
Department of Basic Medical Sciences, College of Medicine, University of Sharjah, Sharjah 27272, UAE
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Department of Pharmaceutical Chemistry and Natural Products, Dubai Pharmacy College, Dubai 19099, UAE
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Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Giza 12613, Egypt
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Sharjah Institute for Medical Research, University of Sharjah, Sharjah 27272, UAE
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Department of Biomedical and Clinical Sciences, Linköping University, SE-581 85 Linköping, Sweden
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Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
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Department of Medical Laboratory Sciences, College of Health Sciences, University of Sharjah, Sharjah 27272, UAE
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Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah 27272, UAE
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(6), 115; https://doi.org/10.3390/ph13060115
Received: 10 May 2020 / Revised: 1 June 2020 / Accepted: 1 June 2020 / Published: 3 June 2020
(This article belongs to the Special Issue Medicinal Plants 2020)
Micromeria fruticosa (L.) Druce subsp. serpyllifolia (Lamiaceae) has been used widely in folk medicine to alleviate various ailments such as abdominal pains, diarrhea, colds, eye infections, heart disorders and wounds. A few reports have confirmed different therapeutic potentialities of its extracts, including the anti-inflammatory, gastroprotective, analgesic, antiobesity and antidiabetic activities. This study aimed to investigate the mechanistic pathway of the antiproliferative activity of the ethanolic extract of M. fruticosa on two different cancer cell lines, namely human breast (mammary carcinoma F7 (MCF-7)) and human colorectal (human colon tumor cells (HCT-116)) cell lines. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) assay, Annexin V-FITC/PI, caspases 8/9 and cell cycle analyses, qRT-PCR and Western blot were used to assess the effect of M. fruticosa on cytotoxicity, apoptosis, cell cycle, cell cycle-related genes and protein expression profiles in MCF-7 and HCT-116. The extract inhibits cell proliferation in a time- and dose-dependent manner. The half-maximal inhibitory concentration (IC50) for both cell lines was found to be 100 μg/mL. Apoptosis induction was confirmed by Annexin V-FITC/PI, that was related to caspases 8 and 9 activities induction. Furthermore, the cell cycle analysis revealed arrest at G2/M phase. The underlying mechanism involved in the G2/M arrest was found to be associated with the downregulation of CDK1, cyclin B1 and survivin that was confirmed by qRT-PCR and Western blotting. View Full-Text
Keywords: Micromeria fruticosa; CDK1; cyclin B1; breast cancer; colon cancer Micromeria fruticosa; CDK1; cyclin B1; breast cancer; colon cancer
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MDPI and ACS Style

El-Huneidi, W.; Shehab, N.G.; Bajbouj, K.; Vinod, A.; El-Serafi, A.; Shafarin, J.; Bou Malhab, L.J.; Abdel-Rahman, W.M.; Abu-Gharbieh, E. Micromeria fruticosa Induces Cell Cycle Arrest and Apoptosis in Breast and Colorectal Cancer Cells. Pharmaceuticals 2020, 13, 115.

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