Next Article in Journal
Vasorelaxant Effects Induced by Red Wine and Pomace Extracts of Magliocco Dolce cv.
Previous Article in Journal
The Impact of Iron Supplementation for Treating Anemia in Patients with Chronic Kidney Disease: Results from Pairwise and Network Meta-Analyses of Randomized Controlled Trials
Open AccessArticle

Crosslinked Fibroin Nanoparticles: Investigations on Biostability, Cytotoxicity, and Cellular Internalization

1
Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
2
PEPITE EA4267, Univ. Bourgogne Franche-Comté, F-25000 Besançon, France
3
The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
4
The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Naresuan University, Phitsanulok 65000, Thailand
*
Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(5), 86; https://doi.org/10.3390/ph13050086
Received: 27 March 2020 / Revised: 19 April 2020 / Accepted: 27 April 2020 / Published: 30 April 2020
(This article belongs to the Section Pharmaceutical Technology)
Recently, crosslinked fibroin nanoparticles (FNP) using the crosslinker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) or the polymer poly(ethylenimine) (PEI) have been developed and showed potentials as novel drug delivery systems. Thus, this study further investigated the biological properties of these crosslinked FNP by labeling them with fluorescein isothiocyanate (FITC) for in vitro studies. All formulations possessed a mean particle size of approximately 300 nm and a tunable zeta potential (−20 to + 30 mV) dependent on the amount/type of crosslinkers. The FITC-bound FNP showed no significant difference in physical properties compared to the blank FNP. They possessed a binding efficacy of 3.3% w/w, and no FITC was released in sink condition up to 8 h. All formulations were colloidal stable in the sheep whole blood. The degradation rate of these FNP in blood could be controlled depending on their crosslink degree. Moreover, no potential toxicity in erythrocytes, Caco-2, HepG2, and 9L cells was noted for all formulations at particle concentrations of < 1 mg/mL. Finally, all FNP were internalized into the Caco-2 cells after 3 h incubation. The uptake rate of the positively charged particles was significantly higher than the negatively charged ones. In summary, the crosslinked FNP were safe and showed high potentials as versatile systems for biomedical applications. View Full-Text
Keywords: fibroin; nanoparticles; crosslink; cellular uptake; biostability fibroin; nanoparticles; crosslink; cellular uptake; biostability
Show Figures

Graphical abstract

MDPI and ACS Style

Pham, D.T.; Saelim, N.; Cornu, R.; Béduneau, A.; Tiyaboonchai, W. Crosslinked Fibroin Nanoparticles: Investigations on Biostability, Cytotoxicity, and Cellular Internalization. Pharmaceuticals 2020, 13, 86.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop