Next Article in Journal
A Novel Protocol Using Small-Scale Spray-Drying for the Efficient Screening of Solid Dispersions in Early Drug Development and Formulation, as a Straight Pathway from Screening to Manufacturing Stages
Next Article in Special Issue
Aptamers as Diagnostic Tools in Cancer
Previous Article in Journal / Special Issue
Nucleic Acid Aptamers Targeting Epigenetic Regulators: An Innovative Therapeutic Option
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessReview
Pharmaceuticals 2018, 11(3), 80;

pH-Control in Aptamer-Based Diagnostics, Therapeutics, and Analytical Applications

Department of Chemistry and Institute of Biochemistry, Carleton University, Ottawa, ON K1S5B6, Canada
Author to whom correspondence should be addressed.
Received: 29 July 2018 / Revised: 20 August 2018 / Accepted: 23 August 2018 / Published: 26 August 2018
(This article belongs to the Special Issue Aptamer-Based Diagnostics and Therapeutics)
Full-Text   |   PDF [1827 KB, uploaded 30 August 2018]   |  


Aptamer binding has been used effectively for diagnostics, in-vivo targeting of therapeutics, and the construction and control of nanomachines. Nanostructures that respond to pH by releasing or changing affinity to a target have also been used for in vivo delivery, and in the construction of sensors and re-usable nanomachines. There are many applications that use aptamers together with pH-responsive materials, notably the targeted delivery of chemotherapeutics. However, the number of reported applications that directly use pH to control aptamer binding is small. In this review, we first discuss the use of aptamers with pH-responsive nanostructures for chemotherapeutic and other applications. We then discuss applications that use pH to denature or otherwise disrupt the binding of aptamers. Finally, we discuss motifs using non-canonical nucleic acid base pairing that can shift conformation in response to pH, followed by an overview of engineered pH-controlled aptamers designed using those motifs. View Full-Text
Keywords: aptamer; pH-responsive; drug delivery; biosensors; DNA nanotechnology; i-motif; triplex aptamer; pH-responsive; drug delivery; biosensors; DNA nanotechnology; i-motif; triplex

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Belleperche, M.; DeRosa, M.C. pH-Control in Aptamer-Based Diagnostics, Therapeutics, and Analytical Applications. Pharmaceuticals 2018, 11, 80.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Pharmaceuticals EISSN 1424-8247 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top