Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials and Equipment
2.1.1. Experimental Materials
2.1.2. Experimental Instruments
2.2. Experimental Method
2.2.1. Preparation of Detection Device
2.2.2. Detection of Affinity Changes
2.2.3. Functionalized Sensing Materials
2.2.4. Signal Acquisition of Biosensor
3. Results and Discussion
3.1. Physical and Optical Microscopic Characterization of the Detection Device and Sensing Area
3.2. Determination of the Optimal Dilution Concentration
3.3. Changes in Electrical Characteristics during Functionalization Process
3.4. Determining the Detection Limit
3.5. Verification of Specificity
3.6. Verification of Reproducibility
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liang, T.; Chen, J.; Yan, R.; Jiang, H.; Li, H. Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor. Sensors 2023, 23, 5820. https://doi.org/10.3390/s23135820
Liang T, Chen J, Yan R, Jiang H, Li H. Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor. Sensors. 2023; 23(13):5820. https://doi.org/10.3390/s23135820
Chicago/Turabian StyleLiang, Ting, Jingfei Chen, Rui Yan, Huaning Jiang, and Hexi Li. 2023. "Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor" Sensors 23, no. 13: 5820. https://doi.org/10.3390/s23135820