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Article

Bio-Interface on Freestanding Nanosheet of Microelectromechanical System Optical Interferometric Immunosensor for Label-Free Attomolar Prostate Cancer Marker Detection

1
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan
2
Electronics Inspired-Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi 441-8580, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Wamadeva Balachandran and Zoltán Fekete
Sensors 2022, 22(4), 1356; https://doi.org/10.3390/s22041356
Received: 7 January 2022 / Revised: 29 January 2022 / Accepted: 6 February 2022 / Published: 10 February 2022
(This article belongs to the Special Issue MEMS Devices for Biomedical Applications)
Various biosensors that are based on microfabrication technology have been developed as point-of-care testing devices for disease screening. The Fabry–Pérot interferometric (FPI) surface-stress sensor was developed to improve detection sensitivity by performing label-free biomarker detection as a nanomechanical deflection of a freestanding membrane to adsorb the molecules. However, chemically functionalizing the freestanding nanosheet with excellent stress sensitivity for selective molecular detection may cause the surface chemical reaction to deteriorate the nanosheet quality. In this study, we developed a minimally invasive chemical functionalization technique to create a biosolid interface on the freestanding nanosheet of a microelectromechanical system optical interferometric surface-stress immunosensor. For receptor immobilization, glutaraldehyde cross-linking on the surface of the amino-functionalized parylene membrane reduced the shape variation of the freestanding nanosheet to 1/5–1/10 of the previous study and achieved a yield of 95%. In addition, the FPI surface-stress sensor demonstrated molecular selectivity and concentration dependence for prostate-specific antigen with a dynamic range of concentrations from 100 ag/mL to 1 µg/mL. In addition, the minimum limit of detection of the proposed sensor was 2,000,000 times lower than that of the conventional nanomechanical cantilevers. View Full-Text
Keywords: microelectromechanical systems; immunosensor; Fabry–Pérot interferometer; surface-stress sensor; biosolid interface; prostate-specific antigen microelectromechanical systems; immunosensor; Fabry–Pérot interferometer; surface-stress sensor; biosolid interface; prostate-specific antigen
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MDPI and ACS Style

Maeda, T.; Kanamori, R.; Choi, Y.-J.; Taki, M.; Noda, T.; Sawada, K.; Takahashi, K. Bio-Interface on Freestanding Nanosheet of Microelectromechanical System Optical Interferometric Immunosensor for Label-Free Attomolar Prostate Cancer Marker Detection. Sensors 2022, 22, 1356. https://doi.org/10.3390/s22041356

AMA Style

Maeda T, Kanamori R, Choi Y-J, Taki M, Noda T, Sawada K, Takahashi K. Bio-Interface on Freestanding Nanosheet of Microelectromechanical System Optical Interferometric Immunosensor for Label-Free Attomolar Prostate Cancer Marker Detection. Sensors. 2022; 22(4):1356. https://doi.org/10.3390/s22041356

Chicago/Turabian Style

Maeda, Tomoya, Ryoto Kanamori, Yong-Joon Choi, Miki Taki, Toshihiko Noda, Kazuaki Sawada, and Kazuhiro Takahashi. 2022. "Bio-Interface on Freestanding Nanosheet of Microelectromechanical System Optical Interferometric Immunosensor for Label-Free Attomolar Prostate Cancer Marker Detection" Sensors 22, no. 4: 1356. https://doi.org/10.3390/s22041356

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