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Article

Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate

Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan
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Academic Editors: Ferdinando Febbraio, Roberto De Michele and Annalisa Rizza
Sensors 2022, 22(3), 1198; https://doi.org/10.3390/s22031198
Received: 21 December 2021 / Revised: 24 January 2022 / Accepted: 31 January 2022 / Published: 4 February 2022
(This article belongs to the Special Issue Genetically-Encoded Fluorescent Sensors in Plants and Fungi)
The NRT1/PTR FAMILY (NPF) in Arabidopsis (Arabidopsis thaliana) plays a major role as a nitrate transporter. The first nitrate transporter activity biosensor NiTrac1 converted the dual-affinity nitrate transceptor NPF6.3 into fluorescence activity sensors. To test whether this approach is transferable to other members of this family, screening for genetically encoded fluorescence transport activity sensor was performed with the member of the NPF family in Arabidopsis. In this study, NPF1.3, an uncharacterized member of NPF in Arabidopsis, was converted into a transporter activity biosensor NiTrac-NPF1.3 that responds specifically to nitrate. The emission ratio change of NiTrac-NPF1.3 triggered by the addition of nitrate reveals the important function of NPF1.3 in nitrate transport in Arabidopsis. A functional analysis of Xenopus laevis oocytes confirmed that NPF1.3 plays a role as a nitrate transporter. This new technology is applicable in plant and medical research. View Full-Text
Keywords: Arabidopsis thaliana; biosensor; fluorescent; transporter Arabidopsis thaliana; biosensor; fluorescent; transporter
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MDPI and ACS Style

Chen, Y.-N.; Ho, C.-H. Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate. Sensors 2022, 22, 1198. https://doi.org/10.3390/s22031198

AMA Style

Chen Y-N, Ho C-H. Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate. Sensors. 2022; 22(3):1198. https://doi.org/10.3390/s22031198

Chicago/Turabian Style

Chen, Yen-Ning, and Cheng-Hsun Ho. 2022. "Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate" Sensors 22, no. 3: 1198. https://doi.org/10.3390/s22031198

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