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Sensors
Volume 21
Issue 5
10.3390/s21051715
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Article

Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect

by
Nada Elgiddawy
1,2,
Shiwei Ren
3,
Wadih Ghattas
1,
Waleed M. A. El Rouby
4,
Ahmed O. El-Gendy
5,6,
Ahmed A. Farghali
4,
Abderrahim Yassar
3 and
Hafsa Korri-Youssoufi
1,*
1
Université Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), ECBB, 91400 Orsay, France
2
Department of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef 62 511, Egypt
3
LPICM, CNRS, Ecole Polytechnique, Institut Polytechnique de Paris, Route de Saclay, 91128 Palaiseau, France
4
Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef 62 511, Egypt
5
Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt
6
Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt
*
Author to whom correspondence should be addressed.
Academic Editor: Benoît Piro
Sensors 2021, 21(5), 1715; https://doi.org/10.3390/s21051715
Received: 13 January 2021 / Revised: 22 February 2021 / Accepted: 22 February 2021 / Published: 2 March 2021
(This article belongs to the Section Biosensors)
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Abstract

Designing therapeutic and sensor materials to diagnose and eliminate bacterial infections remains a significant challenge for active theragnostic nanoprobes. In the present work, fluorescent/electroactive poly(3-hexylthiophene) P3HT nanoparticles (NPs) stabilized with quaternary ammonium salts using cetyltrimethylammonium bromide (CTAB), (CTAB-P3HT NPs) were prepared using a simple mini-emulsion method. The morphology, spectroscopic properties and electronic properties of CTAB-P3HT NPs were characterized by DLS, zeta potential, SEM, TEM, UV-vis spectrophotometry, fluorescence spectroscopy and electrochemical impedance spectroscopy (EIS). In an aqueous solution, CTAB-P3HT NPs were revealed to be uniformly sized, highly fluorescent and present a highly positively charged NP surface with good electroactivity. Dual detection was demonstrated as the binding of the bacteria to NPs could be observed by fluorescence quenching as well as by the changes in EIS. Binding of E. coli to CTAB-P3HT NPs was demonstrated and LODs of 5 CFU/mL and 250 CFU/mL were obtained by relying on the fluorescence spectroscopy and EIS, respectively. The antimicrobial activity of CTAB-P3HT NPs on bacteria and fungi was also studied under dark and nutritive conditions. An MIC and an MBC of 2.5 µg/mL were obtained with E. coli and with S. aureus, and of 0.312 µg/mL with C. albicans. Additionally a good biocompatibility toward normal human cells (WI38) was observed, which opens the way to their possible use as a therapeutic agent. View Full-Text
Keywords: cationic conjugated-polymers; nanoparticles; mini-emulsion; fluorescence; impedance; detection; bacteria; fungi; antimicrobial; biocompatibility cationic conjugated-polymers; nanoparticles; mini-emulsion; fluorescence; impedance; detection; bacteria; fungi; antimicrobial; biocompatibility
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Elgiddawy, N.; Ren, S.; Ghattas, W.; Rouby, W.M.A.E.; El-Gendy, A.O.; Farghali, A.A.; Yassar, A.; Korri-Youssoufi, H. Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect. Sensors 2021, 21, 1715. https://doi.org/10.3390/s21051715

AMA Style

Elgiddawy N, Ren S, Ghattas W, Rouby WMAE, El-Gendy AO, Farghali AA, Yassar A, Korri-Youssoufi H. Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect. Sensors. 2021; 21(5):1715. https://doi.org/10.3390/s21051715

Chicago/Turabian Style

Elgiddawy, Nada, Shiwei Ren, Wadih Ghattas, Waleed M.A.E. Rouby, Ahmed O. El-Gendy, Ahmed A. Farghali, Abderrahim Yassar, and Hafsa Korri-Youssoufi. 2021. "Antimicrobial Activity of Cationic Poly(3-hexylthiophene) Nanoparticles Coupled with Dual Fluorescent and Electrochemical Sensing: Theragnostic Prospect" Sensors 21, no. 5: 1715. https://doi.org/10.3390/s21051715

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