Next Article in Journal
Sensor Development for Corrosion Monitoring of Stainless Steels in H2SO4 Solutions
Previous Article in Journal
A Modular Design for Distributed Measurement of Human–Robot Interaction Forces in Wearable Devices
Previous Article in Special Issue
CellCountCV—A Web-Application for Accurate Cell Counting and Automated Batch Processing of Microscopic Images Using Fully Convolutional Neural Networks
Review

In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives

1
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan
2
Institute for AI and Beyond, The University of Tokyo, Tokyo 113-0033, Japan
3
Center for Information and Neural Networks, National Institute of Information and Communications Technology, Suita City, Osaka 565-0871, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Rawil F. Fakhrullin
Sensors 2021, 21(4), 1448; https://doi.org/10.3390/s21041448
Received: 23 December 2020 / Revised: 12 February 2021 / Accepted: 16 February 2021 / Published: 19 February 2021
(This article belongs to the Special Issue Whole-Cell Biosensor: Recent Advances)
Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp recording techniques. These techniques enable us to record membrane potentials (including action potentials) of individual neurons of not only anesthetized but also actively behaving animals. This whole-cell recording method enables us to reveal how neuronal activities support brain function at the single-cell level. In this review, we introduce previous studies using in vivo patch-clamp recording techniques and recent findings primarily regarding neuronal activities in the hippocampus for behavioral function. We further discuss how we can bridge the gap between electrophysiology and biochemistry. View Full-Text
Keywords: patch-clamp recording; whole-cell recording; rat; mouse; membrane potential; local field potential; hippocampus; neocortex; axon; dendrite patch-clamp recording; whole-cell recording; rat; mouse; membrane potential; local field potential; hippocampus; neocortex; axon; dendrite
Show Figures

Figure 1

MDPI and ACS Style

Noguchi, A.; Ikegaya, Y.; Matsumoto, N. In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives. Sensors 2021, 21, 1448. https://doi.org/10.3390/s21041448

AMA Style

Noguchi A, Ikegaya Y, Matsumoto N. In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives. Sensors. 2021; 21(4):1448. https://doi.org/10.3390/s21041448

Chicago/Turabian Style

Noguchi, Asako, Yuji Ikegaya, and Nobuyoshi Matsumoto. 2021. "In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives" Sensors 21, no. 4: 1448. https://doi.org/10.3390/s21041448

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop