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Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood

1
Siberian Physical-Technical Institute, Tomsk State University, 634050 Tomsk, Russia
2
Federal Research Center, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science, 660036 Krasnoyarsk, Russia
3
Laboratory of Biomolecular and Medical Technologies, Krasnoyarsk State Medical University Named after Prof. V.F. Voyno-Yasenetsky, 660022 Krasnoyarsk, Russia
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Krasnoyarsk Regional Clinical Cancer Center Named after A.I. Kryzhanovsky, 660133 Krasnoyarsk, Russia
5
Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, AB K1N 6N5, Canada
*
Authors to whom correspondence should be addressed.
Academic Editors: José Manuel Díaz-Cruz, Antonio Florido and Núria Serrano
Sensors 2021, 21(23), 7851; https://doi.org/10.3390/s21237851 (registering DOI)
Received: 3 October 2021 / Revised: 12 November 2021 / Accepted: 18 November 2021 / Published: 25 November 2021
We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer’s defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker’s presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics. View Full-Text
Keywords: electrochemical aptasensor; biosensing layer; useful signal; lung cancer; human blood electrochemical aptasensor; biosensing layer; useful signal; lung cancer; human blood
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MDPI and ACS Style

Shabalina, A.V.; Sharko, D.O.; Glazyrin, Y.E.; Bolshevich, E.A.; Dubinina, O.V.; Kim, A.M.; Veprintsev, D.V.; Lapin, I.N.; Zamay, G.S.; Krat, A.V.; Zamay, S.S.; Svetlichnyi, V.A.; Kichkailo, A.S.; Berezovski, M.V. Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood. Sensors 2021, 21, 7851. https://doi.org/10.3390/s21237851

AMA Style

Shabalina AV, Sharko DO, Glazyrin YE, Bolshevich EA, Dubinina OV, Kim AM, Veprintsev DV, Lapin IN, Zamay GS, Krat AV, Zamay SS, Svetlichnyi VA, Kichkailo AS, Berezovski MV. Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood. Sensors. 2021; 21(23):7851. https://doi.org/10.3390/s21237851

Chicago/Turabian Style

Shabalina, Anastasiia V., Darya O. Sharko, Yury E. Glazyrin, Elena A. Bolshevich, Oksana V. Dubinina, Anastasiia M. Kim, Dmitry V. Veprintsev, Ivan N. Lapin, Galina S. Zamay, Alexey V. Krat, Sergey S. Zamay, Valery A. Svetlichnyi, Anna S. Kichkailo, and Maxim V. Berezovski 2021. "Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood" Sensors 21, no. 23: 7851. https://doi.org/10.3390/s21237851

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