Next Article in Journal
Home-Based Sleep Sensor Measurements in an Older Australian Population: Before and during a Pandemic
Previous Article in Journal
A Comparative Evaluation of Inertial Sensors for Gait and Jump Analysis
 
 
Article

Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays

1
Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
2
Department of Analytical Chemistry, Institute of Chemistry, São Paulo State University, Araraquara 14800-060, SP, Brazil
3
Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
4
Institute of Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Benoît Piro
Sensors 2021, 21(18), 5992; https://doi.org/10.3390/s21185992
Received: 3 August 2021 / Revised: 30 August 2021 / Accepted: 31 August 2021 / Published: 7 September 2021
(This article belongs to the Section Biosensors)
This work addresses a method that combines immunomagnetic separation (IMS) and paper-based nucleic acid immunochromatographic assay for the sensitive detection of Mycolicibacterium fortuitum (basonym Mycobacterium fortuitum) In particular, the preconcentration of the bacteria was achieved by using magnetic particles modified with an antibody specific towards mycobacteria. Following the IMS, the bacteria were lysed, and the genome was amplified by double-tagging PCR, using a set of primers specific for the 16S rRNA gene for Mycobacterium. During the amplification, the amplicons were labeled with biotin and digoxigenin tags. Moreover, a comparative study of paper-based immunochromatographic platforms, relying on vertical and lateral flow and on the use of streptavidin gold nanoparticles as a signal generating system, was also performed. The visual readout was achieved when the gold-modified amplicons were captured by the anti-DIG antibody in the test line. The analytical performance of both methods, nucleic acid vertical flow (NAVF) and nucleic acid lateral flow (NALF), is also discussed. Although NALF showed lower limit of detections (LODs), both NALF and NAVF combined with IMS were able to detect the required LOD in hemodialysis water, becoming two promising and useful techniques for the rapid screening of water supplies in hemodialysis centers, to prevent the exposure of immunosuppressed patients to contaminated sources. View Full-Text
Keywords: rapid diagnostic tests; lateral flow assay; vertical flow assay; gold nanoparticles; mycobacteria; double-tagging PCR rapid diagnostic tests; lateral flow assay; vertical flow assay; gold nanoparticles; mycobacteria; double-tagging PCR
Show Figures

Figure 1

MDPI and ACS Style

Ben Aissa, A.; Araújo, B.; Julián, E.; Boldrin Zanoni, M.V.; Pividori, M.I. Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays. Sensors 2021, 21, 5992. https://doi.org/10.3390/s21185992

AMA Style

Ben Aissa A, Araújo B, Julián E, Boldrin Zanoni MV, Pividori MI. Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays. Sensors. 2021; 21(18):5992. https://doi.org/10.3390/s21185992

Chicago/Turabian Style

Ben Aissa, Alejandra, Barbara Araújo, Esther Julián, Maria Valnice Boldrin Zanoni, and María Isabel Pividori. 2021. "Immunomagnetic Separation Improves the Detection of Mycobacteria by Paper-Based Lateral and Vertical Flow Immunochromatographic Assays" Sensors 21, no. 18: 5992. https://doi.org/10.3390/s21185992

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop