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Recent Advancements in Enzyme-Based Lateral Flow Immunoassays
Article

Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen

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Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
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Department of Chemistry Education, Seoul National University, Seoul 05029, Korea
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BioSquare Inc., Seongnam 13620, Korea
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Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA
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Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305, USA
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Department of Chemical and Biological Engineering, Hanbat National University, Daejeon 34158, Korea
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School of International Engineering and Science, Jeonbuk National University, Jeonju 54896, Korea
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Department of Urology, Seoul National University Bundang Hospital, Seongnam 13620, Korea
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Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Korea
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Authors to whom correspondence should be addressed.
Academic Editor: Fabio Di Nardo
Sensors 2021, 21(12), 4099; https://doi.org/10.3390/s21124099
Received: 6 May 2021 / Revised: 10 June 2021 / Accepted: 12 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Lateral Flow Immunoassay: Advances and Applications)
Prostate-specific antigen (PSA) is the best-known biomarker for early diagnosis of prostate cancer. For prostate cancer in particular, the threshold level of PSA <4.0 ng/mL in clinical samples is an important indicator. Quick and easy visual detection of the PSA level greatly helps in early detection and treatment of prostate cancer and reducing mortality. In this study, we developed optimized silica-coated silver-assembled silica nanoparticles (SiO2@[email protected]2 NPs) that were applied to a visual lateral flow immunoassay (LFIA) platform for PSA detection. During synthesis, the ratio of silica NPs to silver nitrate changed, and as the synthesized NPs exhibited distinct UV spectra and colors, most optimized SiO2@[email protected]2 NPs showed the potential for early prostate cancer diagnosis. The PSA detection limit of our LFIA platform was 1.1 ng/mL. By applying each SiO2@[email protected]2 NP to the visual LFIA platform, optimized SiO2@[email protected]2 NPs were selected in the test strip, and clinical samples from prostate cancer patients were successfully detected as the boundaries of non-specific binding were clearly seen and the level of PSA was <4 ng/mL, thus providing an avenue for quick prostate cancer diagnosis and early treatment. View Full-Text
Keywords: silica nanoparticles; prostate-specific antigen; lateral flow immunoassay silica nanoparticles; prostate-specific antigen; lateral flow immunoassay
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MDPI and ACS Style

Kim, H.-M.; Kim, J.; Bock, S.; An, J.; Choi, Y.-S.; Pham, X.-H.; Cha, M.G.; Seong, B.; Kim, W.; Kim, Y.-H.; Song, H.; Kim, J.-W.; Park, S.-m.; Lee, S.H.; Rho, W.-Y.; Lee, S.; Jeong, D.H.; Lee, H.-Y.; Jun, B.-H. Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen. Sensors 2021, 21, 4099. https://doi.org/10.3390/s21124099

AMA Style

Kim H-M, Kim J, Bock S, An J, Choi Y-S, Pham X-H, Cha MG, Seong B, Kim W, Kim Y-H, Song H, Kim J-W, Park S-m, Lee SH, Rho W-Y, Lee S, Jeong DH, Lee H-Y, Jun B-H. Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen. Sensors. 2021; 21(12):4099. https://doi.org/10.3390/s21124099

Chicago/Turabian Style

Kim, Hyung-Mo, Jaehi Kim, Sungje Bock, Jaehyun An, Yun-Sik Choi, Xuan-Hung Pham, Myeong G. Cha, Bomi Seong, Wooyeon Kim, Yoon-Hee Kim, Hobeom Song, Jung-Won Kim, Seung-min Park, Sang H. Lee, Won-Yeop Rho, Sangchul Lee, Dae H. Jeong, Ho-Young Lee, and Bong-Hyun Jun. 2021. "Silver-Assembled Silica Nanoparticles in Lateral Flow Immunoassay for Visual Inspection of Prostate-Specific Antigen" Sensors 21, no. 12: 4099. https://doi.org/10.3390/s21124099

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