An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma
Abstract
:1. Introduction
2. Experimental
2.1. Materials and Reagents
2.2. Biological Material
2.3. Procedures
2.3.1. Antibody Immobilization
2.3.2. SPRi Measurements
3. Results and Discussion
3.1. Optimization of Antibody Concentration
3.2. Dependence of the Analytical Signal on HE4 Concentration. Calibration Graph
3.3. Precision and Recovery. Limit of Detection
3.4. Specificity of the Developed Biosensor
3.5. Validation of the Developed Biosensor
3.6. Example of Diagnostic Application
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Appendix A.1. Materials
Appendix A.2. Apparatus and Chip Architecture
Appendix A.3. Optimization of Concentration of Rabbit Polyclonal Antibody against HE 4
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Added (pM) | Found (pM) | SD (pM) | RSD (%) | Recovery (%) |
---|---|---|---|---|
11.4 | 11.6 | 0.68 | 6.0 | 101.8 |
45.5 | 46.5 | 4.5 | 9.9 | 102.1 |
114 | 118 | 11 | 9.6 | 103.5 |
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Szymanska, B.; Lukaszewski, Z.; Zelazowska-Rutkowska, B.; Hermanowicz-Szamatowicz, K.; Gorodkiewicz, E. An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma. Sensors 2021, 21, 3567. https://doi.org/10.3390/s21103567
Szymanska B, Lukaszewski Z, Zelazowska-Rutkowska B, Hermanowicz-Szamatowicz K, Gorodkiewicz E. An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma. Sensors. 2021; 21(10):3567. https://doi.org/10.3390/s21103567
Chicago/Turabian StyleSzymanska, Beata, Zenon Lukaszewski, Beata Zelazowska-Rutkowska, Kinga Hermanowicz-Szamatowicz, and Ewa Gorodkiewicz. 2021. "An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma" Sensors 21, no. 10: 3567. https://doi.org/10.3390/s21103567
APA StyleSzymanska, B., Lukaszewski, Z., Zelazowska-Rutkowska, B., Hermanowicz-Szamatowicz, K., & Gorodkiewicz, E. (2021). An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma. Sensors, 21(10), 3567. https://doi.org/10.3390/s21103567