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Open AccessArticle

A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment

1
Biophysics and Nanoscience Centre, DEB, Università degli Studi della Tuscia, 01100 Viterbo, Italy
2
CNR-IMM Institute for Microelectronics and Microsystems, Via Monteroni, University Campus, Bld. A/3, 73100 Lecce, Italy
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(21), 6364; https://doi.org/10.3390/s20216364
Received: 28 September 2020 / Revised: 5 November 2020 / Accepted: 5 November 2020 / Published: 8 November 2020
(This article belongs to the Section Biosensors)
The concentration of wild-type tumour suppressor p53wt in cells and blood has a clinical significance for early diagnosis of some types of cancer. We developed a disposable, label-free, field-effect transistor-based immunosensor (BioFET), able to detect p53wt in physiological buffer solutions, over a wide concentration range. Microfabricated, high-purity gold electrodes were used as single-use extended gates (EG), which avoid direct interaction between the transistor gate and the biological solution. Debye screening, which normally hampers target charge effect on the FET gate potential and, consequently, on the registered FET drain-source current, at physiological ionic strength, was overcome by incorporating a biomolecule-permeable polymer layer on the EG electrode surface. Determination of an unknown p53wt concentration was obtained by calibrating the variation of the FET threshold voltage versus the target molecule concentration in buffer solution, with a sensitivity of 1.5 ± 0.2 mV/decade. The BioFET specificity was assessed by control experiments with proteins that may unspecifically bind at the EG surface, while 100pM p53wt concentration was established as limit of detection. This work paves the way for fast and highly sensitive tools for p53wt detection in physiological fluids, which deserve much interest in early cancer diagnosis and prognosis. View Full-Text
Keywords: p53; EGFET; immunosensors; BioFET p53; EGFET; immunosensors; BioFET
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MDPI and ACS Style

Baldacchini, C.; Montanarella, A.F.; Francioso, L.; Signore, M.A.; Cannistraro, S.; Bizzarri, A.R. A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment. Sensors 2020, 20, 6364. https://doi.org/10.3390/s20216364

AMA Style

Baldacchini C, Montanarella AF, Francioso L, Signore MA, Cannistraro S, Bizzarri AR. A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment. Sensors. 2020; 20(21):6364. https://doi.org/10.3390/s20216364

Chicago/Turabian Style

Baldacchini, Chiara; Montanarella, Antonino F.; Francioso, Luca; Signore, Maria A.; Cannistraro, Salvatore; Bizzarri, Anna R. 2020. "A Reliable BioFET Immunosensor for Detection of p53 Tumour Suppressor in Physiological-Like Environment" Sensors 20, no. 21: 6364. https://doi.org/10.3390/s20216364

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