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Multiplexed Remote SPR Detection of Biological Interactions through Optical Fiber Bundles

1
Univ. Grenoble Alpes, CEA, CNRS, SyMMES, 38000 Grenoble, France
2
Univ. Bordeaux, INP-Bordeaux, ISM, CNRS UMR5255, 33607 Pessac, France
3
CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(2), 511; https://doi.org/10.3390/s20020511
Received: 19 December 2019 / Revised: 8 January 2020 / Accepted: 14 January 2020 / Published: 16 January 2020
(This article belongs to the Special Issue Fibre-Optic Biosensing)
The development of sensitive methods for in situ detection of biomarkers is a real challenge to bring medical diagnosis a step forward. The proof-of-concept of a remote multiplexed biomolecular interaction detection through a plasmonic optical fiber bundle is demonstrated here. The strategy relies on a fiber optic biosensor designed from a 300 µm diameter bundle composed of 6000 individual optical fibers. When appropriately etched and metallized, each optical fiber exhibits specific plasmonic properties. The surface plasmon resonance phenomenon occurring at the surface of each fiber enables to measure biomolecular interactions, through the changes of the retro-reflected light intensity due to light/plasmon coupling variations. The functionalization of the microstructured bundle by multiple protein probes was performed using new polymeric 3D-printed microcantilevers. Such soft cantilevers allow for immobilizing the probes in micro spots, without damaging the optical microstructures nor the gold layer. We show here the potential of this device to perform the multiplexed detection of two different antibodies with limits of detection down to a few tenths of nanomoles per liter. This tool, adapted for multiparametric, real-time, and label free monitoring is minimally invasive and could then provide a useful platform for in vivo targeted molecular analysis. View Full-Text
Keywords: optical fiber; biosensor; surface plasmon resonance; SPR; label-free; multiplexed detection; biomolecular detection; functionalization; microstructuration optical fiber; biosensor; surface plasmon resonance; SPR; label-free; multiplexed detection; biomolecular detection; functionalization; microstructuration
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Desmet, C.; Vindas, K.; Alvarado Meza, R.; Garrigue, P.; Voci, S.; Sojic, N.; Maziz, A.; Courson, R.; Malaquin, L.; Leichle, T.; Buhot, A.; Roupioz, Y.; Leroy, L.; Engel, E. Multiplexed Remote SPR Detection of Biological Interactions through Optical Fiber Bundles. Sensors 2020, 20, 511.

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