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Letter

Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye

by 2,3,†, 1,†, 1, 1, 1, 1, 2,4 and 1,*
1
School of Stomatology, Lanzhou University, Lanzhou 730000, China
2
National Center for Nanoscience and Nanotechnology, University of Chinese Academy of Sciences, Beijing 100190, China
3
School of Medicine, Tulane University, New Orleans, LA 70112, USA
4
Department of Pharmacology Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, TX 75390, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2020, 20(17), 4831; https://doi.org/10.3390/s20174831
Received: 30 July 2020 / Revised: 14 August 2020 / Accepted: 19 August 2020 / Published: 27 August 2020
(This article belongs to the Special Issue Lab-on-a-Chip and Microfluidic Sensors)
We have developed an alginate hydrogel-embedded capillary sensor (AHCS) for naked eye-based quantification of immunoassay. Alkaline phosphatase (ALP) can modulate gel-sol transformation to increase the permeability of Cu2+-cross-linked alginate hydrogel film in the AHCS, followed by solution exchange into the capillary. Through measuring the length of the liquid phase of the microfluidics in the capillary at a given time, the concentration of the ALP could be quantified with the naked eye. Since ALP is widely applied as a signal reporter for immunoassays, the AHCS could easily accommodate conventional immune sensing platforms. We justify the practicality of AHCS with hepatitis B virus surface antigen (HBsAg) in serum samples and got comparable results with commercialized immunoassay. This AHCS is easy to make and use, effective in cost, and robust in quantification with the naked eye, showing great promise for next generation point-of-care testing. View Full-Text
Keywords: immunoassay; POCT; alginate hydrogel; capillary microfluidics immunoassay; POCT; alginate hydrogel; capillary microfluidics
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MDPI and ACS Style

Zheng, W.; Gao, C.; Shen, L.; Qu, C.; Zhang, X.; Yang, L.; Feng, Q.; Tang, R. Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye. Sensors 2020, 20, 4831. https://doi.org/10.3390/s20174831

AMA Style

Zheng W, Gao C, Shen L, Qu C, Zhang X, Yang L, Feng Q, Tang R. Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye. Sensors. 2020; 20(17):4831. https://doi.org/10.3390/s20174831

Chicago/Turabian Style

Zheng, Wenshu, Cen Gao, Liheng Shen, Chang Qu, Xuan Zhang, Lu Yang, Qiang Feng, and Rongbing Tang. 2020. "Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye" Sensors 20, no. 17: 4831. https://doi.org/10.3390/s20174831

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