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Article

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

1
Department of Physical Chemistry, Higher Technical School of Industrial Engineering, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, Spain
2
Department of Physical Chemistry and Institute of Electrochemistry, University of Alicante, 03690 San Vicente del Raspeig, Spain
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(16), 4489; https://doi.org/10.3390/s20164489
Received: 15 July 2020 / Revised: 7 August 2020 / Accepted: 9 August 2020 / Published: 11 August 2020
(This article belongs to the Special Issue Nanoparticles-Based Sensors)
Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H2O2. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM−1 cm−2), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors. View Full-Text
Keywords: glucose; glucose oxidase; enzymatic biosensor; poly(Azure A); platinum nanoparticles; activated screen-printed carbon electrodes glucose; glucose oxidase; enzymatic biosensor; poly(Azure A); platinum nanoparticles; activated screen-printed carbon electrodes
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MDPI and ACS Style

Jiménez-Fiérrez, F.; González-Sánchez, M.I.; Jiménez-Pérez, R.; Iniesta, J.; Valero, E. Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A). Sensors 2020, 20, 4489. https://doi.org/10.3390/s20164489

AMA Style

Jiménez-Fiérrez F, González-Sánchez MI, Jiménez-Pérez R, Iniesta J, Valero E. Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A). Sensors. 2020; 20(16):4489. https://doi.org/10.3390/s20164489

Chicago/Turabian Style

Jiménez-Fiérrez, Francisco, María I. González-Sánchez, Rebeca Jiménez-Pérez, Jesús Iniesta, and Edelmira Valero. 2020. "Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)" Sensors 20, no. 16: 4489. https://doi.org/10.3390/s20164489

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