Next Article in Journal
Vital Sign Detection during Large-Scale and Fast Body Movements Based on an Adaptive Noise Cancellation Algorithm Using a Single Doppler Radar Sensor
Previous Article in Journal
Compact Current Reference Circuits with Low Temperature Drift and High Compliance Voltage
Open AccessArticle

Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis

1
School of Natural Sciences, Bangor University, post code Bangor, Gwynedd, Wales LL57 2UW, UK
2
Department of Chemistry, Xi’an Jiaotong-Liverpool University, 111 Ren’ai Road, Suzhou Industrial Park, Suzhou 215123, China
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(15), 4182; https://doi.org/10.3390/s20154182
Received: 15 June 2020 / Revised: 19 July 2020 / Accepted: 21 July 2020 / Published: 28 July 2020
(This article belongs to the Section Biosensors)
Population extended life expectancy has significantly increased the risk of septic shock in an ageing population. Sepsis affects roughly 20 million people every year, resulting in over 11 million deaths. The need for faster more accurate diagnostics and better management is therefore paramount in the fight to prevent these avoidable deaths. Here we report the development of a POC device with the ability to identify a broad range of pathogens on a lateral flow platform. Namely Gram-positive and Gram-negative bacteria. The simple to use laboratory device has the potential to be automated, thus enabling an operator to carry out solid-phase lysis and room temperature RPA in situ, providing accurate results in hours rather than days. Results show there is a potential for a fully automated device in which concepts described in this paper can be integrated into a lateral flow device. View Full-Text
Keywords: sepsis; lateral flow immunoassay; solid phase RPA; point-of-care; bacterial infections sepsis; lateral flow immunoassay; solid phase RPA; point-of-care; bacterial infections
Show Figures

Figure 1

MDPI and ACS Style

Heeroma, A.J.; Gwenin, C. Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis. Sensors 2020, 20, 4182. https://doi.org/10.3390/s20154182

AMA Style

Heeroma AJ, Gwenin C. Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis. Sensors. 2020; 20(15):4182. https://doi.org/10.3390/s20154182

Chicago/Turabian Style

Heeroma, Alice J.; Gwenin, Christopher. 2020. "Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis" Sensors 20, no. 15: 4182. https://doi.org/10.3390/s20154182

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop