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A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode

by Jing Chen 1,2,3, Qiwen Yu 1, Wei Fu 1, Xing Chen 1,3, Quan Zhang 4, Shurong Dong 5,6,7, Hang Chen 1,3,* and Shaomin Zhang 1,2,3,5,7
1
Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China
2
Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou 310027, China
3
Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal of China, Zhejiang University, Hangzhou 310027, China
4
Neural Systems Group, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
5
Zhejiang Laboratory, Hangzhou 310027, China
6
Key Laboratory of Micro-nano Electronic Devices and Smart Systems of Zhejiang Province, College of Information Science and Electronic Engineering, Zhejiang University, Hangzhou 310027, China
7
MOE Frontier Science Center for Brain Research and Brain-Machine Integration, Zhejiang University, Hangzhou 310027, China
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(10), 2924; https://doi.org/10.3390/s20102924
Received: 4 May 2020 / Revised: 18 May 2020 / Accepted: 20 May 2020 / Published: 21 May 2020
(This article belongs to the Section Biosensors)
A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM–40 µM), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research. View Full-Text
Keywords: microbiosensor; glutamate; glutamate oxidase; Prussian blue nanocubes; chitosan; amperometry microbiosensor; glutamate; glutamate oxidase; Prussian blue nanocubes; chitosan; amperometry
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MDPI and ACS Style

Chen, J.; Yu, Q.; Fu, W.; Chen, X.; Zhang, Q.; Dong, S.; Chen, H.; Zhang, S. A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode. Sensors 2020, 20, 2924.

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