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Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor

1
Institut für Mikrosensoren, -Aktoren und -Systeme, Universität Bremen, 28359 Bremen, Germany
2
Bremerhavener Institut für Angewandte Molekularbiologie, Hochschule Bremerhaven, 27568 Bremerhaven, Germany
*
Author to whom correspondence should be addressed.
This paper is an extended version of our paper published in “Prada, J.; Cordes, C.; Harms, C.; Lang, W. Design and manufacturing of a disposable, Cyclo-Olefin Copolymer, microfluidic biosensor. In Proceedings of the Eurosensors 2018, Graz, Austria, 9–12 September 2018”.
These authors contributed equally to this work.
Sensors 2019, 19(5), 1178; https://doi.org/10.3390/s19051178
Received: 29 January 2019 / Revised: 26 February 2019 / Accepted: 1 March 2019 / Published: 7 March 2019
(This article belongs to the Special Issue Eurosensors 2018 Selected Papers)
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PDF [1985 KB, uploaded 7 March 2019]
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Abstract

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems. View Full-Text
Keywords: biosensors; Cyclo-Olefin Copolymer; cell heat-lysis; resistive temperature sensor; RNA hybridization; fluorescence detection biosensors; Cyclo-Olefin Copolymer; cell heat-lysis; resistive temperature sensor; RNA hybridization; fluorescence detection
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Prada, J.; Cordes, C.; Harms, C.; Lang, W. Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor . Sensors 2019, 19, 1178.

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