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Open AccessArticle

SERS-Based Immunoassays for the Detection of Botulinum Toxins A and B Using Magnetic Beads

Department of Chemistry, Chung-Ang University, Seoul 06974, Korea
Department of Bionano Technology, Hanyang University, Ansan 426-791, Korea
Division of High-risk Pathogens, Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Chungju 28159, Korea
Author to whom correspondence should be addressed.
Sensors 2019, 19(19), 4081;
Received: 5 September 2019 / Revised: 17 September 2019 / Accepted: 18 September 2019 / Published: 21 September 2019
(This article belongs to the Special Issue Sensors for Hazardous Material Detection)
Rapid and sensitive detection of botulinum neurotoxins (BoNTs) is important for immediate treatment with proper antitoxins. However, it is difficult to detect BoNTs at the acute phase of infection, owing to its rarity and ambiguous symptoms. To resolve this problem, we developed a surface-enhanced Raman scattering (SERS)-based immunoassay technique for the rapid and sensitive detection of BoNTs. Magnetic beads and SERS nanotags as capture substrates and detection probes, respectively, and Nile Blue A (NBA) and malachite green isothiocyanate (MGITC) as Raman reporter molecules were used for the detection of two different types of BoNTs (types A and B), respectively. The corresponding limits of detection (LODs) were determined as 5.7 ng/mL (type A) and 1.3 ng/mL (type B). Total assay time, including that for immunoreaction, washing, and detection, was less than 2 h. View Full-Text
Keywords: surface-enhanced Raman scattering (SERS); botulinum neurotoxins; bioterrorism surface-enhanced Raman scattering (SERS); botulinum neurotoxins; bioterrorism
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Kim, K.; Choi, N.; Jeon, J.H.; Rhie, G.-E.; Choo, J. SERS-Based Immunoassays for the Detection of Botulinum Toxins A and B Using Magnetic Beads. Sensors 2019, 19, 4081.

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